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TitleToward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold
Journal, issue, pagesProtein Sci., Vol. 17, Page 1175-1187, Year 2008
Publish dateMar 11, 2007 (structure data deposition date)
AuthorsTereshko, V. / Uysal, S. / Koide, A. / Margalef, K. / Koide, S. / Kossiakoff, A.A.
External linksProtein Sci. / PubMed:18445622
MethodsX-ray diffraction
Resolution1.1 - 2.5 Å
Structure data

PDB-2p42:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.8A resolution: SE3-mono-2 crystal form with three se-met sites (M34, M51, M83) in vhh scaffold
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2p43:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.65A resolution: SE3-mono-1 crystal form with three se-met sites (M34, M51, M83) in vhh scaffold
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-2p44:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.8A resolution: SE5A-mono-1 crystal form with five se-met sites (M34, M51, F68M, M83, L86M) in vhh scaffold
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-2p45:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.1A resolution: SE5B-ORTHO-1 crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-2p46:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 2.5A resolution: se5b-ortho-2 crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-2p47:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 2.5A resolution: SE5B-TRI crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-2p48:
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 2.3A resolution: SE5B-tetra crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-ZN:
Unknown entry

Source
  • camelus dromedarius (Arabian camel)
  • bos taurus (cattle)
KeywordsHYDROLASE/IMMUNE SYSTEM / SEMET PHASING / CAMELID SINGLE-DOMAIN ANTIBODY / VHH / CAB-RN05 / RNASE A / YEAST SURFACE DISPLAY. / HYDROLASE-IMMUNE SYSTEM COMPLEX / YEAST SURFACE DISPLAY / HYDROLASE

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