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Structure paper

TitleCaught in the act II: Visualization of an intermediate in the DNA base-flipping pathway induced by the adenine-specific DNA methyltransferase M.TaqI
Journal, issue, pagesTo be Published
Publish dateOct 26, 2006 (structure data deposition date)
AuthorsLenz, T. / Scheidig, A.J. / Weinhold, E.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.9 - 2.1 Å
Structure data

PDB-2np6:
Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-2np7:
Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target position and pyrrolo-dC at the target base partner position
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-NEA:
5'-DEOXY-5'-[2-(AMINO)ETHYLTHIO]ADENOSINE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • thermus aquaticus (bacteria)
KeywordsTRANSFERASE/DNA / DNA / DNA methyltransferase / abasic site analog / base flipping / nucleotide flipping / TRANSFERASE-DNA COMPLEX / target base partner / pyrrolo-dC

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