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TitleSelf-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.
Journal, issue, pagesJ Biol Chem, Vol. 282, Issue 6, Page 4035-4044, Year 2007
Publish dateFeb 9, 2007
AuthorsSteven Johnson / Pietro Roversi / Marianela Espina / Andrew Olive / Janet E Deane / Susan Birket / Terry Field / William D Picking / Ariel J Blocker / Edouard E Galyov / Wendy L Picking / Susan M Lea /
PubMed AbstractBacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. ...Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.
External linksJ Biol Chem / PubMed:17077085 / PubMed Central
MethodsX-ray diffraction
Resolution2.1 - 3.1 Å
Structure data

PDB-2ixr:
BipD of Burkholderia Pseudomallei
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-2j0n:
A proteolytically truncated form of Shigella Flexneri IpaD
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-2j0o:
Shigella Flexneri IpaD
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-2j9t:
BipD of Burkholderia Pseudomallei
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-2jaa:
SeMet substituted Shigella Flexneri Ipad
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-BO3:
BORIC ACID

ChemComp-FLC:
CITRATE ANION

Source
  • burkholderia pseudomallei (bacteria)
  • shigella flexneri 2a str. 301 (bacteria)
  • shigella flexneri (bacteria)
KeywordsTOXIN / BURKHOLDERIA PSEUDOMALLEI / BIPD / TTSS / T3SS / TYPE 3 SECRETION SYSTEM / CELL INVASION / IPAD / PLASMID / VIRULENCE / SHIGELLA FLEXNERI / TYPE III SECRETION / INVASIN / SEMET

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