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TitleHigh-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations.
Journal, issue, pagesEMBO J, Vol. 25, Issue 18, Page 4187-4194, Year 2006
Publish dateSep 20, 2006
AuthorsMasahide Kikkawa / Nobutaka Hirokawa /
PubMed AbstractKinesin is an ATP-driven microtubule (MT)-based motor fundamental to organelle transport. Although a number of kinesin crystal structures have been solved, the structural evidence for coupling ...Kinesin is an ATP-driven microtubule (MT)-based motor fundamental to organelle transport. Although a number of kinesin crystal structures have been solved, the structural evidence for coupling between the bound nucleotide and the conformation of kinesin is elusive. In addition, the structural basis of the MT-induced ATPase activity of kinesin is not clear because of the absence of the MT in the structure. Here, we report cryo-electron microscopy structures of the monomeric kinesin KIF1A-MT complex in two nucleotide states at about 10 A resolution, sufficient to reveal the secondary structure. These high-resolution maps visualized clear structural changes that suggest a mechanical pathway from the nucleotide to the neck linker via the motor core rotation. In addition, new nucleotide binding pocket conformations are observed that are different from X-ray crystallographic structures; it is closed in the 5'-adenylyl-imidodiphosphate state, but open in the ADP state. These results suggest a structural model of biased diffusion movement of monomeric kinesin motor.
External linksEMBO J / PubMed:16946706 / PubMed Central
MethodsEM (helical sym.)
Resolution10 - 11 Å
Structure data

PDB-2hxf:
KIF1A head-microtubule complex structure in amppnp-form
Method: ELECTRON MICROSCOPY / Resolution: 10.0 Å

PDB-2hxh:
KIF1A head-microtubule complex structure in adp-form
Method: ELECTRON MICROSCOPY / Resolution: 11.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • mus musculus (house mouse)
  • sus scrofa (pig)
KeywordsTRANSPORT PROTEIN / microtubule-based motor

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