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TitleCapsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM.
Journal, issue, pagesStructure, Vol. 14, Issue 11, Page 1655-1665, Year 2006
Publish dateJan 16, 2007
AuthorsLu Gan / Jeffrey A Speir / James F Conway / Gabriel Lander / Naiqian Cheng / Brian A Firek / Roger W Hendrix / Robert L Duda / Lars Liljas / John E Johnson /
PubMed AbstractMaturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major ...Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
External linksStructure / PubMed:17098191
MethodsX-ray diffraction / EM (single particle)
Resolution3.8 - 7.5 Å
Structure data

PDB-2frp:
Bacteriophage HK97 Expansion Intermediate IV
Method: X-RAY DIFFRACTION / Resolution: 7.5 Å

PDB-2fs3:
Bacteriophage HK97 K169Y Head I
Method: X-RAY DIFFRACTION / Resolution: 4.2 Å

PDB-2fsy:
Bacteriophage HK97 Pepsin-treated Expansion Intermediate IV
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

PDB-2ft1:
Bacteriophage HK97 Head II
Method: X-RAY DIFFRACTION / Resolution: 3.9 Å

PDB-2fte:
Bacteriophage HK97 Expansion Intermediate IV
Method: ELECTRON MICROSCOPY

Source
  • enterobacteria phage hk97 (virus)
KeywordsVIRUS / Bacteriophage / HK97 / Capsid Protein / Expansion Intermediate / icosahedral VIRUS / capsid / mature capsid / VIRUS/VIRAL PROTEIN / VIRUS-VIRAL PROTEIN COMPLEX

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