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TitleNucleotide flips determine the specificity of the Ecl18kI restriction endonuclease
Journal, issue, pagesEmbo J., Vol. 25, Page 2219-2229, Year 2006
Publish dateJan 18, 2006 (structure data deposition date)
AuthorsBochtler, M. / Szczepanowski, R.H. / Tamulaitis, G. / Grazulis, S. / Czapinska, H. / Manakova, E. / Siksnys, V.
External linksEmbo J. / PubMed:16628220
MethodsX-ray diffraction
Resolution1.7 - 2 Å
Structure data

PDB-2fqz:
Metal-depleted Ecl18kI in complex with uncleaved DNA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2gb7:
Metal-depleted Ecl18kI in complex with uncleaved, modified DNA
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • enterobacter cloacae (bacteria)
KeywordsHydrolase/DNA / ECL18KI-DNA COMPLEX / TYPE II RESTRICTION ENDONUCLEASE / NUCLEOTIDE FLIPPING / BASE EXTRUSION / Hydrolase-DNA COMPLEX

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