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TitleInsights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor.
Journal, issue, pagesNew Phytol, Vol. 174, Page 90-100, Year 2007
Publish dateJul 20, 2005 (structure data deposition date)
AuthorsVerhaest, M. / Lammens, W. / Le Roy, K. / De Ranter, C.J. / Van Laere, A. / Rabijns, A. / Van den Ende, W.
External linksNew Phytol / PubMed:17335500
MethodsX-ray diffraction
Resolution2.5 - 3.27 Å
Structure data

PDB-2add:
Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with sucrose
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-2ade:
Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with fructose
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-2aey:
Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with 2,5 dideoxy-2,5-immino-D-mannitol
Method: X-RAY DIFFRACTION / Resolution: 3.27 Å

PDB-2aez:
Crystal structure of fructan 1-exohydrolase IIa (E201Q) from Cichorium intybus in complex with 1-kestose
Method: X-RAY DIFFRACTION / Resolution: 3.05 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-FRU:
beta-D-fructofuranose

ChemComp-DQQ:
2,5-DIDEOXY-2,5-IMINO-D-MANNITOL

Source
  • cichorium intybus (chicory)
KeywordsHYDROLASE / five fold beta propeller

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