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TitleDesigner gene therapy using an Escherichia coli purine nucleoside phosphorylase/prodrug system.
Journal, issue, pagesChem. Biol., Vol. 10, Page 1173-1181, Year 2003
Publish dateMar 23, 2003 (structure data deposition date)
AuthorsBennett, E.M. / Anand, R. / Allan, P.W. / Hassan, A.E. / Hong, J.S. / Levasseur, D.N. / McPherson, D.T. / Parker, W.B. / Secrist, J.A. / Sorscher, E.J. ...Bennett, E.M. / Anand, R. / Allan, P.W. / Hassan, A.E. / Hong, J.S. / Levasseur, D.N. / McPherson, D.T. / Parker, W.B. / Secrist, J.A. / Sorscher, E.J. / Townes, T.M. / Waud, W.R. / Ealick, S.E.
External linksChem. Biol. / PubMed:14700625
MethodsX-ray diffraction
Resolution2.2 - 2.7 Å
Structure data

PDB-1otx:
Purine Nucleoside Phosphorylase M64V mutant
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-1oty:
Native PNP +ALLO
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-1ou4:
Native PNP +Talo
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-1oum:
M64V PNP +Talo
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1ov6:
M64V PNP + ALLO
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-1ovg:
M64V PNP +MePdr
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-6MP:
6-METHYLPURINE

ChemComp-TAL:
9-(6-DEOXY-ALPHA-L-TALOFURANOSYL)-6-METHYLPURINE

ChemComp-DBM:
9-(6-DEOXY-BETA-D-ALLOFURANOSYL)-6-METHYLPURINE

ChemComp-MDR:
9-(2-DEOXY-BETA-D-RIBOFURANOSYL)-6-METHYLPURINE

Source
  • escherichia coli (E. coli)
KeywordsTRANSFERASE / empty / PNP / GLYCOSYLTRANSFERASE / Native / Talo / M64V / mutant PNP / ALLO / Mepdr

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