Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle.
Journal, issue, pages	J Struct Biol, Vol. 138, Issue 1-2, Page 92-9104, Year 2002
Publish date	Jul 18, 2003
Authors	Li Fan Chen / Hanspeter Winkler / Michael K Reedy / Mary C Reedy / Kenneth A Taylor /
PubMed Abstract	Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), ...Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.
External links	J Struct Biol / PubMed:12160705
Methods	EM (tomography)
Resolution	70 Å
Structure data	PDB-1m8q: Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1mvw: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o18: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o19: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1a: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1b: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1c: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1d: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1e: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1f: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å PDB-1o1g: MOLECULAR MODELS OF AVERAGED RIGOR CROSSBRIDGES FROM TOMOGRAMS OF INSECT FLIGHT MUSCLE Method: ELECTRON MICROSCOPY / Resolution: 70 Å
Source	gallus gallus (chicken) oryctolagus cuniculus (rabbit)
Keywords	CONTRACTILE PROTEIN / Actin-myosin complex in situ in muscle