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Structure paper

TitleStructural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin.
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 59, Page 1567-1573, Year 2003
Publish dateOct 30, 2002 (structure data deposition date)
AuthorsLe Trong, I. / Freitag, S. / Klumb, L.A. / Chu, V. / Stayton, P.S. / Stenkamp, R.E.
External linksActa Crystallogr. ,Sect. D / PubMed:12925786
MethodsX-ray diffraction
Resolution1.53 - 2.18 Å
Structure data

PDB-1n43:
Streptavidin Mutant N23A with biotin at 1.89A
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-1n4j:
STREPTAVIDIN MUTANT N23A AT 2.18A
Method: X-RAY DIFFRACTION / Resolution: 2.18 Å

PDB-1n7y:
STREPTAVIDIN MUTANT N23E AT 1.96A
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-1n9m:
Streptavidin Mutant S27A with Biotin at 1.6A Resolution
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1n9y:
Streptavidin Mutant S27A at 1.5A Resolution
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-1nbx:
Streptavidin Mutant Y43A at 1.70A Resolution
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-1nc9:
STREPTAVIDIN MUTANT Y43A WITH IMINOBIOTIN AT 1.8A RESOLUTION
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-1ndj:
Streptavidin Mutant Y43F with Biotin at 1.81A Resolution
Method: X-RAY DIFFRACTION / Resolution: 1.81 Å

Chemicals

ChemComp-BTN:
BIOTIN / Biotin

ChemComp-HOH:
WATER / Water

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-IMI:
2-IMINOBIOTIN

Source
  • streptomyces avidinii (bacteria)
KeywordsBiotin-binding protein / tetramer / homotetramer

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