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-Structure paper
Title | Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. |
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Journal, issue, pages | Cell, Vol. 108, Issue 5, Page 717-725, Year 2002 |
Publish date | Mar 8, 2002 |
Authors | Richard J Kuhn / Wei Zhang / Michael G Rossmann / Sergei V Pletnev / Jeroen Corver / Edith Lenches / Christopher T Jones / Suchetana Mukhopadhyay / Paul R Chipman / Ellen G Strauss / Timothy S Baker / James H Strauss / |
PubMed Abstract | The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus ...The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus core, within a lipid bilayer, has a less-ordered structure than the external, icosahedral scaffold of 90 glycoprotein E dimers. The three E monomers per icosahedral asymmetric unit do not have quasiequivalent symmetric environments. Difference maps indicate the location of the small membrane protein M relative to the overlaying scaffold of E dimers. The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane. |
External links | Cell / PubMed:11893341 / PubMed Central |
Methods | EM (single particle) |
Resolution | 24 Å |
Structure data | PDB-1k4r: |
Source |
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Keywords | VIRUS / Flavivirus / Flaviviridae / Dengue virus / glycoprotein E from tick-borne encephalitis virus / Icosahedral virus |