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TitleHigh Resolution X-Ray Crystallography Shows that Ascorbate is a Cofactor for Myrosinase and Substitutes for the Function of the Catalytic Base
Journal, issue, pagesJ. Biol. Chem., Vol. 275, Page 39385-, Year 2000
Publish dateJul 10, 2000 (structure data deposition date)
AuthorsBurmeister, W.P. / Cottaz, S. / Rollin, P. / Vasella, A. / Henrissat, B.
External linksJ. Biol. Chem. / PubMed:10978344
MethodsX-ray diffraction
Resolution1.2 - 1.65 Å
Structure data

PDB-1e4m:
MYROSINASE FROM SINAPIS ALBA
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

PDB-1e6q:
MYROSINASE FROM SINAPIS ALBA with the bound transition state analogue gluco-tetrazole
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-1e6s:
MYROSINASE FROM SINAPIS ALBA with bound gluco-hydroximolactam and sulfate
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-1e6x:
MYROSINASE FROM SINAPIS ALBA with a bound transition state analogue,D-glucono-1,5-lactone
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1e70:
2-F-glucosylated MYROSINASE FROM SINAPIS ALBA
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-1e71:
MYROSINASE FROM SINAPIS ALBA with bound ascorbate
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-1e72:
Myrosinase from Sinapis alba with bound gluco-hydroximolactam and sulfate or ascorbate
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-1e73:
2-F-glucosylated MYROSINASE FROM SINAPIS ALBA with bound L-ascorbate
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-ZN:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NTZ:
NOJIRIMYCINE TETRAZOLE

ChemComp-GOX:
(2S,3S,4R,5R)-6-(HYDROXYAMINO)-2-(HYDROXYMETHYL)-2,3,4,5-TETRAHYDROPYRIDINE-3,4,5-TRIOL

ChemComp-LGC:
D-glucono-1,5-lactone

ChemComp-G2F:
2-deoxy-2-fluoro-alpha-D-glucopyranose

ChemComp-ASC:
ASCORBIC ACID / medication*YM

Source
  • sinapis alba (white mustard)
KeywordsHYDROLASE / FAMILY 1 GLYCOSYL HYDROLASE / GLUCOSINOLATE / TIM BARREL / D-GLUCONO-1 / 5-LACTONE / TRANSITION STATE / INHIBITOR / TRANSITION STATE ANALOGUE / MYROSINASE / GLUCOSYL ENZYME / ACTIVATION / ASCORBATE

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