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TitleAn anti-CRISPR protein disables type V Cas12a by acetylation.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 4, Page 308-314, Year 2019
Publish dateApr 1, 2019
AuthorsLiyong Dong / Xiaoyu Guan / Ningning Li / Fan Zhang / Yuwei Zhu / Kuan Ren / Ling Yu / Fengxia Zhou / Zhifu Han / Ning Gao / Zhiwei Huang /
PubMed AbstractPhages use anti-CRISPR proteins to deactivate the CRISPR-Cas system. The mechanisms for the inhibition of type I and type II systems by anti-CRISPRs have been elucidated. However, it has remained ...Phages use anti-CRISPR proteins to deactivate the CRISPR-Cas system. The mechanisms for the inhibition of type I and type II systems by anti-CRISPRs have been elucidated. However, it has remained unknown how the type V CRISPR-Cas12a (Cpf1) system is inhibited by anti-CRISPRs. Here we identify the anti-CRISPR protein AcrVA5 and report the mechanisms by which it inhibits CRISPR-Cas12a. Our structural and biochemical data show that AcrVA5 functions as an acetyltransferase to modify Moraxella bovoculi (Mb) Cas12a at Lys635, a residue that is required for recognition of the protospacer-adjacent motif. The AcrVA5-mediated modification of MbCas12a results in complete loss of double-stranded DNA (dsDNA)-cleavage activity. In contrast, the Lys635Arg mutation renders MbCas12a completely insensitive to inhibition by AcrVA5. A cryo-EM structure of the AcrVA5-acetylated MbCas12a reveals that Lys635 acetylation provides sufficient steric hindrance to prevent dsDNA substrates from binding to the Cas protein. Our study reveals an unprecedented mechanism of CRISPR-Cas inhibition and suggests an evolutionary arms race between phages and bacteria.
External linksNat Struct Mol Biol / PubMed:30936526
MethodsEM (single particle) / X-ray diffraction
Resolution2.052 - 3.6 Å
Structure data

9742

6iv6

EMDB-9742, PDB-6iv6:
Cryo-EM structure of AcrVA5-acetylated MbCas12a in complex with crRNA
Method: EM (single particle) / Resolution: 3.6 Å

PDB-6iuf:
Crystal structure of Anti-CRISPR protein AcrVA5
Method: X-RAY DIFFRACTION / Resolution: 2.052 Å

Chemicals

ChemComp-ACO:
ACETYL COENZYME *A / Acetyl-CoA

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

Source
  • moraxella bovoculi (bacteria)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / enzyme / IMMUNE SYSTEM/RNA / IMMUNE SYSTEM-RNA complex

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