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TitleT = 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display.
Journal, issue, pagesViruses, Vol. 10, Issue 12, Year 2018
Publish dateNov 26, 2018
AuthorsZhiqing Zhang / Maozhou He / Shimeng Bai / Feng Zhang / Jie Jiang / Qingbing Zheng / Shuangquan Gao / Xiaodong Yan / Shaowei Li / Ying Gu / Ningshao Xia /
PubMed AbstractThe HIV-1 mature capsid (CA) assumes an amorphous, fullerene conical configuration due to its high flexibility. How native CA self-assembles is still unclear despite having well-defined structures of ...The HIV-1 mature capsid (CA) assumes an amorphous, fullerene conical configuration due to its high flexibility. How native CA self-assembles is still unclear despite having well-defined structures of its pentamer and hexamer building blocks. Here we explored the self-assembly of an engineered capsid protein built through artificial disulfide bonding (CA N21C/A22C) and determined the structure of one fraction of the globular particles. CA N21C/A22C was found to self-assemble into particles in relatively high ionic solutions. These particles contained disulfide-bonding hexamers as determined via non-reducing SDS-PAGE, and exhibited two major components of 57.3 S and 80.5 S in the sedimentation velocity assay. Particles had a globular morphology, approximately 40 nm in diameter, in negative-staining TEM. Through cryo-EM 3-D reconstruction, we determined a novel T = 4 icosahedral structure of CA, comprising 12 pentamers and 30 hexamers at 25 Å resolution. We engineered the HIV-1 V3 loop to the CA particles, and found the resultant particles resembled the morphology of their parental particles in TEM, had a positive reaction with V3-specific neutralizing antibodies, and conferred neutralization immunogenicity in mice. Our results shed light on HIV CA assembly and provide a particulate CA for epitope display.
External linksViruses / PubMed:30486318 / PubMed Central
MethodsEM (single particle)
Resolution25.0 Å
Structure data

EMDB-9733:
A T=4 icosahedral structure of HIV-1 capsid
Method: EM (single particle) / Resolution: 25.0 Å

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