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TitleStructural bases for F plasmid conjugation and F pilus biogenesis in .
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 28, Page 14222-14227, Year 2019
Publish dateJul 9, 2019
AuthorsBo Hu / Pratick Khara / Peter J Christie /
PubMed AbstractBacterial conjugation systems are members of the large type IV secretion system (T4SS) superfamily. Conjugative transfer of F plasmids residing in the was first reported in the 1940s, yet the ...Bacterial conjugation systems are members of the large type IV secretion system (T4SS) superfamily. Conjugative transfer of F plasmids residing in the was first reported in the 1940s, yet the architecture of F plasmid-encoded transfer channel and its physical relationship with the F pilus remain unknown. We visualized F-encoded structures in the native bacterial cell envelope by in situ cryoelectron tomography (CryoET). Remarkably, F plasmids encode four distinct structures, not just the translocation channel or channel-pilus complex predicted by prevailing models. The F1 structure is composed of distinct outer and inner membrane complexes and a connecting cylinder that together house the envelope-spanning translocation channel. The F2 structure is essentially the F1 complex with the F pilus attached at the outer membrane (OM). Remarkably, the F3 structure consists of the F pilus attached to a thin, cell envelope-spanning stalk, whereas the F4 structure consists of the pilus docked to the OM without an associated periplasmic density. The traffic ATPase TraC is configured as a hexamer of dimers at the cytoplasmic faces of the F1 and F2 structures, where it respectively regulates substrate transfer and F pilus biogenesis. Together, our findings present architectural renderings of the DNA conjugation or "mating" channel, the channel-pilus connection, and unprecedented pilus basal structures. These structural snapshots support a model for biogenesis of the F transfer system and allow for detailed comparisons with other structurally characterized T4SSs.
External linksProc Natl Acad Sci U S A / PubMed:31239340 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution22.7 - 23.9 Å
Structure data

EMDB-9344:
Type IV secretion machine
Method: EM (subtomogram averaging) / Resolution: 22.7 Å

EMDB-9347:
type iv secretion machine
Method: EM (subtomogram averaging) / Resolution: 23.9 Å

Source
  • Escherichia coli (E. coli)

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