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| Title | Flaviviruses have imperfect icosahedral symmetry. |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 115, Issue 45, Page 11608-11612, Year 2018 |
| Publish date | Nov 6, 2018 |
 Authors | Matthew D Therkelsen / Thomas Klose / Frank Vago / Wen Jiang / Michael G Rossmann / Richard J Kuhn /  |
| PubMed Abstract | Flaviviruses assemble initially in an immature, noninfectious state and undergo extensive conformational rearrangements to generate mature virus. Previous cryo-electron microscopy (cryo-EM) ...Flaviviruses assemble initially in an immature, noninfectious state and undergo extensive conformational rearrangements to generate mature virus. Previous cryo-electron microscopy (cryo-EM) structural studies of flaviviruses assumed icosahedral symmetry and showed the concentric organization of the external glycoprotein shell, the lipid membrane, and the internal nucleocapsid core. We show here that when icosahedral symmetry constraints were excluded in calculating the cryo-EM reconstruction of an immature flavivirus, the nucleocapsid core was positioned asymmetrically with respect to the glycoprotein shell. The core was positioned closer to the lipid membrane at the proximal pole, and at the distal pole, the outer glycoprotein spikes and inner membrane leaflet were either perturbed or missing. In contrast, in the asymmetric reconstruction of a mature flavivirus, the core was positioned concentric with the glycoprotein shell. The deviations from icosahedral symmetry demonstrated that the core and glycoproteins have varied interactions, which likely promotes viral assembly and budding. |
 External links | Proc Natl Acad Sci U S A / PubMed:30348794 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 6.4 - 35.0 Å |
| Structure data |  EMDB-8982:  EMDB-8983:  EMDB-8984:  EMDB-8985: |
| Source |
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Kunjin virus (STRAIN MRM61C)