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TitleStructures of the Human HCN1 Hyperpolarization-Activated Channel.
Journal, issue, pagesCell, Vol. 168, Issue 1-2, Page 111-120.e11, Year 2017
Publish dateJan 12, 2017
AuthorsChia-Hsueh Lee / Roderick MacKinnon /
PubMed AbstractHyperpolarization-activated cyclic nucleotide-gated (HCN) channels underlie the control of rhythmic activity in cardiac and neuronal pacemaker cells. In HCN, the polarity of voltage dependence is ...Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels underlie the control of rhythmic activity in cardiac and neuronal pacemaker cells. In HCN, the polarity of voltage dependence is uniquely reversed. Intracellular cyclic adenosine monophosphate (cAMP) levels tune the voltage response, enabling sympathetic nerve stimulation to increase the heart rate. We present cryo-electron microscopy structures of the human HCN channel in the absence and presence of cAMP at 3.5 Å resolution. HCN channels contain a K channel selectivity filter-forming sequence from which the amino acids create a unique structure that explains Na and K permeability. The voltage sensor adopts a depolarized conformation, and the pore is closed. An S4 helix of unprecedented length extends into the cytoplasm, contacts the C-linker, and twists the inner helical gate shut. cAMP binding rotates cytoplasmic domains to favor opening of the inner helical gate. These structures advance understanding of ion selectivity, reversed polarity gating, and cAMP regulation in HCN channels.
External linksCell / PubMed:28086084 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.51 Å
Structure data

8511

5u6o

EMDB-8511, PDB-5u6o:
Structure of the human HCN1 hyperpolarization-activated cyclic nucleotide-gated ion channel
Method: EM (single particle) / Resolution: 3.5 Å

8512

5u6p

EMDB-8512, PDB-5u6p:
Structure of the human HCN1 hyperpolarization-activated cyclic nucleotide-gated ion channel in complex with cAMP
Method: EM (single particle) / Resolution: 3.51 Å

Chemicals

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE / Cyclic adenosine monophosphate

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / pacemaker ion channel

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