Yi-Wei Chang / Andreas Kjær / Davi R Ortega / Gabriela Kovacikova / John A Sutherland / Lee A Rettberg / Ronald K Taylor / Grant J Jensen /
PubMed Abstract
Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal ...Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal body. Based on pilin features, T4P are classified into type IVa pili (T4aP) and type IVb pili (T4bP). T4aP are more widespread and are involved in cell motility, DNA transfer, host predation and electron transfer. T4bP are less prevalent and are mainly found in enteropathogenic bacteria, where they play key roles in host colonization. Following similar work on T4aP machines, here we use electron cryotomography to reveal the three-dimensional in situ structure of a T4bP machine in its piliated and non-piliated states. The specific machine we analyse is the Vibrio cholerae toxin-coregulated pilus machine (TCPM). Although only about half of the components of the TCPM show sequence homology to components of the previously analysed Myxococcus xanthus T4aP machine (T4aPM), we find that their structures are nevertheless remarkably similar. Based on homologies with components of the M. xanthus T4aPM and additional reconstructions of TCPM mutants in which the non-homologous proteins are individually deleted, we propose locations for all eight TCPM components within the complex. Non-homologous proteins in the T4aPM and TCPM are found to form similar structures, suggesting new hypotheses for their functions and evolutionary histories.
EMDB-3792: Electron cryotomogram of Vibrio cholerae O395 N1 Method: EM (tomography)
EMDB-8492: Subtomogram average of the non-piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8493: Subtomogram average of the non-piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8494: Subtomogram average of the piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8495: Subtomogram average of the piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8496: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpS knockout (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8497: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpS knockout (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8498: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpB knockout (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8499: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpB knockout (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8500: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpD knockout (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8501: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpD knockout (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8502: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpR knockout (aligning OM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
EMDB-8503: Subtomogram average of the non-piliated toxin-coregulated pilus machine in Vibrio cholerae cells with tcpR knockout (aligning IM-parts) Method: EM (subtomogram averaging) / Resolution: 50.0 Å
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Vibrio cholerae (bacteria)
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Vibrio cholerae (bacteria)