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Title | Structure of the STRA6 receptor for retinol uptake. |
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Journal, issue, pages | Science, Vol. 353, Issue 6302, Year 2016 |
Publish date | Aug 26, 2016 |
![]() | Yunting Chen / Oliver B Clarke / Jonathan Kim / Sean Stowe / Youn-Kyung Kim / Zahra Assur / Michael Cavalier / Raquel Godoy-Ruiz / Desiree C von Alpen / Chiara Manzini / William S Blaner / Joachim Frank / Loredana Quadro / David J Weber / Lawrence Shapiro / Wayne A Hendrickson / Filippo Mancia / ![]() |
PubMed Abstract | Vitamin A homeostasis is critical to normal cellular function. Retinol-binding protein (RBP) is the sole specific carrier in the bloodstream for hydrophobic retinol, the main form in which vitamin A ...Vitamin A homeostasis is critical to normal cellular function. Retinol-binding protein (RBP) is the sole specific carrier in the bloodstream for hydrophobic retinol, the main form in which vitamin A is transported. The integral membrane receptor STRA6 mediates cellular uptake of vitamin A by recognizing RBP-retinol to trigger release and internalization of retinol. We present the structure of zebrafish STRA6 determined to 3.9-angstrom resolution by single-particle cryo-electron microscopy. STRA6 has one intramembrane and nine transmembrane helices in an intricate dimeric assembly. Unexpectedly, calmodulin is bound tightly to STRA6 in a noncanonical arrangement. Residues involved with RBP binding map to an archlike structure that covers a deep lipophilic cleft. This cleft is open to the membrane, suggesting a possible mode for internalization of retinol through direct diffusion into the lipid bilayer. |
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Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.739 - 3.9 Å |
Structure data | EMDB-8315, PDB-5sy1: ![]() PDB-5k8q: |
Chemicals | ![]() ChemComp-CA: ![]() ChemComp-IMD: ![]() ChemComp-HOH: ![]() ChemComp-CLR: |
Source |
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![]() | METAL BINDING PROTEIN / Calmodulin / STRA6 / peptide complex / unique fold / MEMBRANE PROTEIN/CALCIUM BINDING PROTEIN / Vitamin A / retinol / membrane / MEMBRANE PROTEIN-CALCIUM BINDING PROTEIN complex |