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TitleF-Type Pyocin Versus Phage λ Tail: Conserved Hub, Divergent Fibers.
Journal, issue, pagesAdv Sci (Weinh), Page e77063, Year 2026
Publish dateAug 10, 2026
AuthorsZhiwei Gu / Yufan Xie / Lanxin Wang / Luyan Ma / Xiaofei Ge / Jiawei Wang /
PubMed AbstractBacteriocins are ribosomally synthesized antimicrobial peptides or proteins that offer an alternative to conventional antibiotics against multidrug-resistant pathogens. Phage tail-like bacteriocins ...Bacteriocins are ribosomally synthesized antimicrobial peptides or proteins that offer an alternative to conventional antibiotics against multidrug-resistant pathogens. Phage tail-like bacteriocins (tailocins) are classified into rigid R-type and flexible F-type variants. While R-type pyocins from Pseudomonas aeruginosa are well-characterized, F-type pyocins remain poorly understood, especially with respect to the molecular mechanisms for their Gram-negative bactericidal activity. Here, we report cryo-electron microscopy structures of the F-type pyocin from P. aeruginosa ATCC 15442 at 2.29-3.26 Å resolution, encompassing three modular components: the tail cap, tail tip, and tail fiber. Structural comparisons with bacteriophage λ reveal a conserved tail tip architecture, including the baseplate hub proteins, distal tail protein, tail assembly protein, and tape measure protein. Unexpectedly, we identify three trimeric side fibers that attach not to the distal tail protein, as in canonical systems, but to the α-helical shaft of the central fiber, indicating a previously unrecognized attachment mode. The receptor-binding domain of the side fiber shares structural similarity with LPS-recognizing domains of R-type pyocins. Together, these results define the structural basis of F-type pyocin assembly and host recognition, reveal conserved and unique features relative to phage λ, and provide a framework for engineering tailocins as precision antimicrobials against drug-resistant P. aeruginosa.
External linksAdv Sci (Weinh) / PubMed:42573577 / PubMed Central
MethodsEM (single particle)
Resolution2.29 - 3.26 Å
Structure data

80401

25vd

EMDB-80401, PDB-25vd:
Cap of F2-pyocin
Method: EM (single particle) / Resolution: 2.55 Å

80402

25ve

EMDB-80402, PDB-25ve:
side fiber of F2-pyocin
Method: EM (single particle) / Resolution: 2.78 Å

80403

25vf

EMDB-80403, PDB-25vf:
F2-pyocin tail Tip
Method: EM (single particle) / Resolution: 2.29 Å

80404

25vj

EMDB-80404, PDB-25vj:
tail fiber of F2-pyocin
Method: EM (single particle) / Resolution: 3.26 Å

80538

26ci

EMDB-80538, PDB-26ci:
F2-pyocin FNIII and AHS
Method: EM (single particle) / Resolution: 2.63 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsVIRAL PROTEIN / pyocin / phage / cap / tail F2 pyocin tip / F2-pyocin tail tip AHS FNIII

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