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TitleDual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateJun 1, 2018
AuthorsVera P Pisareva / Andrey V Pisarev / Israel S Fernández /
PubMed AbstractCo-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its ...Co-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its structural genes in the late stage of infection. Ribosome hijacking is achieved by a sophisticated use of molecular mimicry to tRNA and mRNA, employed to manipulate intrinsically dynamic components of the ribosome. Binding and translocation through the ribosome is required for this IRES to initiate translation. We report two structures, solved by single particle electron cryo-microscopy (cryoEM), of a double translocated CrPV-IRES with aminoacyl-tRNA in the peptidyl site (P site) of the ribosome. CrPV-IRES adopts a previously unseen conformation, mimicking the acceptor stem of a canonical E site tRNA. The structures suggest a mechanism for the positioning of the first aminoacyl-tRNA shared with the distantly related Hepatitis C Virus IRES.
External linksElife / PubMed:29856316 / PubMed Central
MethodsEM (single particle)
Resolution3.2 Å
Structure data

7834

6d90

EMDB-7834, PDB-6d90:
Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1.
Method: EM (single particle) / Resolution: 3.2 Å

7836

6d9j

EMDB-7836, PDB-6d9j:
Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1.
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • Rabbit (rabbit)
  • homo sapiens (human)
  • escherichia coli (E. coli)
  • cricket paralysis virus
KeywordsRIBOSOME / mammalian / CrPV IRES

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