[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleThe I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility.
Journal, issue, pagesMol Biol Cell, Vol. 29, Issue 9, Page 1048-1059, Year 2018
Publish dateMay 1, 2018
AuthorsGang Fu / Qian Wang / Nhan Phan / Paulina Urbanska / Ewa Joachimiak / Jianfeng Lin / Dorota Wloga / Daniela Nicastro /
PubMed AbstractMotile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their ...Motile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their regulatory mechanisms are not well understood. The tether and tether head (T/TH) complex was hypothesized to provide mechanical feedback during ciliary beating because it links the motor domains of the regulatory I1 dynein to the ciliary doublet microtubule. Combining genetic and biochemical approaches with cryoelectron tomography, we identified FAP44 and FAP43 (plus the algae-specific, FAP43-redundant FAP244) as T/TH components. WT-mutant comparisons revealed that the heterodimeric T/TH complex is required for the positional stability of the I1 dynein motor domains, stable anchoring of CK1 kinase, and proper phosphorylation of the regulatory IC138-subunit. T/TH also interacts with inner dynein arm d and radial spoke 3, another important motility regulator. The T/TH complex is a conserved regulator of I1 dynein and plays an important role in the signaling pathway that is critical for normal ciliary motility.
External linksMol Biol Cell / PubMed:29514928 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution30.0 - 40.0 Å
Structure data

EMDB-7481:
The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (3736 axonemal repeats) of isolated Chlamydomonas wild type cilia
Method: EM (subtomogram averaging) / Resolution: 30.0 Å

EMDB-7486:
The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1885 axonemal repeats) of isolated Chlamydomonas fap44 mutant cilia
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-7487:
The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1711 axonemal repeats) of isolated Chlamydomonas fap43 mutant cilia (axonemal repeats missing the tether and tether head complex were not included)
Method: EM (tomography) / Resolution: 37.0 Å

EMDB-7488:
The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1622 axonemal repeats) of isolated Tetrahymena wild type cilia
Method: EM (tomography) / Resolution: 33.0 Å

EMDB-7489:
The tether and tether head complex and I1 dynein complex region extracted from the cryo-electron tomography and subtomographic average (1150 axonemal repeats) of isolated Tetrahymena FAP43KO mutant cilia
Method: EM (tomography) / Resolution: 39.0 Å

Source
  • Chlamydomonas reinhardtii (plant)
  • Tetrahymena thermophila (eukaryote)

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more