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Structure paper

TitleRNA catalysis emerges from dynamic structural ensembles
Journal, issue, pagesNature, Year 2026
Publish dateNov 26, 2025 (structure data deposition date)
AuthorsDegenhardt MFS / Degenhardt HF / Bhoge BA / Lee Y-T / Yu P / Zhang J / Deme JC / Stagno JR / Wang Y-X
External linksNature / Search PubMed
MethodsEM (single particle)
Resolution2.86 - 3.9 Å
Structure data

EMDB-74074, PDB-9zdv:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 51
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-74131, PDB-9zf2:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 1
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74132, PDB-9zf3:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 48
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74133, PDB-9zf5:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 49
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-74137, PDB-9zf9:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 55
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-74140, PDB-9zfc:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 2
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74141, PDB-9zfd:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 3
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-74195, PDB-9zgm:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 4
Method: EM (single particle) / Resolution: 3.01 Å

EMDB-74196, PDB-9zgn:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 5
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74197, PDB-9zgo:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 6
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74198, PDB-9zgp:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 7
Method: EM (single particle) / Resolution: 2.86 Å

EMDB-74199, PDB-9zgq:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 8
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74200, PDB-9zgr:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 9
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-74201, PDB-9zgs:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 10
Method: EM (single particle) / Resolution: 2.91 Å

EMDB-74202, PDB-9zgt:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 11
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74203, PDB-9zgu:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 12
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-74204, PDB-9zgv:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 14
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-74205, PDB-9zgw:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 13
Method: EM (single particle) / Resolution: 2.98 Å

EMDB-74206, PDB-9zgx:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 15
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74207, PDB-9zgy:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 16
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74208, PDB-9zgz:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 17
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74209, PDB-9zh0:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 18
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74213, PDB-9zh1:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 19
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74215, PDB-9zh2:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 20
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74223, PDB-9zh5:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 21
Method: EM (single particle) / Resolution: 2.94 Å

EMDB-74224, PDB-9zh7:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 22
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-74226, PDB-9zh9:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 23
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74227, PDB-9zha:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 24
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-74228, PDB-9zhb:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 25
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74229, PDB-9zhc:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 26
Method: EM (single particle) / Resolution: 2.93 Å

EMDB-74230, PDB-9zhd:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 27
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74231, PDB-9zhe:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 28
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74232, PDB-9zhf:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 29
Method: EM (single particle) / Resolution: 2.88 Å

EMDB-74235, PDB-9zhg:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 30
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74237, PDB-9zhi:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 31
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74238, PDB-9zhj:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 32
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-74239, PDB-9zhk:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 33
Method: EM (single particle) / Resolution: 3.03 Å

EMDB-74261, PDB-9zi5:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 47
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-74263, PDB-9zi6:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 52
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-74264, PDB-9zi7:
Cryo-EM structure of RNase P RNA from Geobacillus stearothermophilus, conformer 54
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-74284, PDB-9ziu:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 16
Method: EM (single particle) / Resolution: 3.45 Å

EMDB-74285, PDB-9ziv:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 17
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-74286, PDB-9ziw:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 18
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-74287, PDB-9zix:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 20
Method: EM (single particle) / Resolution: 3.68 Å

EMDB-74288, PDB-9ziy:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 21
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-74289, PDB-9ziz:
Cryo-EM structure of Geobacillus stearothermophilus RNase P holoenzyme in 1 mM Mg2+, conformer 19
Method: EM (single particle) / Resolution: 3.24 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • geobacillus stearothermophilus (bacteria)
KeywordsRNA / RNase P RNA / Geobacillus stearothermophilus / catalytic RNA / cryo-EM structure / conformational heterogeneity / RNA/HYDROLASE / ribozyme / RNase P / RNA-Hydrolase complex / RNase P.

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