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TitleVisualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart.
Journal, issue, pagesNat Commun, Year 2026
Publish dateMay 16, 2026
AuthorsPeter L Ducos / Alexander T Duckworth / Kenneth A Satyshur / James L Keck / Timothy Grant /
PubMed AbstractReplication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome ...Replication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome complex, comprising the PriA, PriB, and DnaT proteins, onto an abandoned replication fork. Here, we present two distinct preprimosome structures. One represents an intermediate stage in preprimosome assembly with a single DnaT C-terminal domain (DnaT) bound to PriA/PriB/DNA. The second captures the mature preprimosome, in which filamentation of multiple DnaT molecules catalyzes the handoff of the single-stranded lagging-strand DNA from PriB to DnaT. The DnaT N-terminal domain forms a separate, independent oligomer in the mature structure. Taken together, our results detail the molecular mechanisms underlying replication restart initiation and regulation and suggest mechanistic similarities between DnaT and the canonical initiator protein DnaA.
External linksNat Commun / PubMed:42140935
MethodsEM (single particle)
Resolution3.22 - 3.6 Å
Structure data

74008

9zbt

EMDB-74008, PDB-9zbt:
Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome
Method: EM (single particle) / Resolution: 3.22 Å

74009

9zbu

EMDB-74009, PDB-9zbu:
Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli k-12 (bacteria)
  • synthetic construct (others)
KeywordsREPLICATION / DNA replication / helicase / oligomer / DNA repair

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