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TitleIdentification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.
Journal, issue, pagesStructure, Year 2026
Publish dateJul 1, 2026
AuthorsShishir Pandey / Nathaniel Burman / William S Henriques / Tanner Wiegand / Trevor Zahl / Hannah Nyquist / Tanner Spreeuw / Murat Buyukyoruk / Blake Wiedenheft /
PubMed AbstractCas7-family proteins form the scaffolds of multi-subunit CRISPR RNA-guided surveillance complexes. To explore how Cas7 diversification expands CRISPR function, we identified Cas7 fusion proteins ...Cas7-family proteins form the scaffolds of multi-subunit CRISPR RNA-guided surveillance complexes. To explore how Cas7 diversification expands CRISPR function, we identified Cas7 fusion proteins linked to diverse accessory domains, including a type III-B variant (III-Bv) in which a Cas7 homolog (Cmr1) is fused to the MntA antitoxin and encoded adjacent to a HEPN-family toxin. Structures reveal that the core Cas proteins assemble into a stable surveillance complex in the absence of crRNA, whereas incorporation of the Cmr1-MntA fusion is crRNA-dependent. Target RNA recognition triggers conformational changes that expose the Cas10 cyclase active site and promote cyclic oligoadenylate synthesis. Biochemical analyses show that the CRISPR-associated MntA is enzymatically active and AMPylates the associated HEPN protein. Together, these findings establish the structural basis for assembly of a type III-Bv surveillance complex containing an enzymatically active toxin-antitoxin module.
External linksStructure / PubMed:42385699 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.0 Å
Structure data

73877

9z7q

EMDB-73877, PDB-9z7q:
Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to a crRNA
Method: EM (single particle) / Resolution: 3.0 Å

73882

9z7v

EMDB-73882, PDB-9z7v:
Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to target RNA with complementary PFS
Method: EM (single particle) / Resolution: 3.0 Å

73883

9z7x

EMDB-73883, PDB-9z7x:
Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to target RNA with non-complementary PFS
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • dissulfurispira thermophila (bacteria)
  • escherichia phage ms2 (virus)
KeywordsIMMUNE SYSTEM/RNA / CRISPR / complex / crRNA / IMMUNE SYSTEM / IMMUNE SYSTEM-RNA complex / target / RNA

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