EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational landscape of HIV-1 Env from closed to fully open.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年2月24日
著者	Jiayan Cui / Zi Jie Lin / Sukanya Ghosh / Jianqiu Du / Roopak Sadeesh / David B Weiner / Jesper Pallesen /
PubMed 要旨	The molecular mechanism of HIV-1 entry into host cells is governed by dynamic conformational changes to its envelope glycoprotein (Env), which are triggered by the engagement of the host receptor CD4 ...The molecular mechanism of HIV-1 entry into host cells is governed by dynamic conformational changes to its envelope glycoprotein (Env), which are triggered by the engagement of the host receptor CD4 and coreceptors. Structural insights into these transitions have been advanced by cryo-electron tomography (cryo-ET), resolving Env structures in closed and multifarious open states within native membranes, and by cryo-electron microscopy (cryo-EM), which has provided atomic details of these states. In this study, we determine cryo-EM structures of soluble native-like Env in complex with antibody 3BC315, antibody b12, CD4, or a combination of 3BC315 and b12, capturing previously uncharacterized conformational states. Observing enhanced 3BC315 binding occupancy in the presence of b12, we investigate the cooperativity of these antibodies using mass photometry and neutralization assays. Integrating these states with the literature, we establish a classification framework for symmetric and asymmetric Env states, categorizing by their degree of openness and stepwise structural rearrangements. Our findings refine the mechanistic understanding of HIV-1 Env dynamics and provide a structural roadmap for targeting dynamic Env states to develop more potent vaccines and immunotherapies.
リンク	Nat Commun / PubMed:41735302
手法	EM (単粒子)
解像度	2.91 - 3.88 Å
構造データ	49236 9nbt EMDB-49236, PDB-9nbt: AMC008 v4.2 SOSIP Env trimer in complex with VRC01 and 35O22 Fabs 手法: EM (単粒子) / 解像度: 2.91 Å 49238 9nby EMDB-49238, PDB-9nby: AMC008 v4.2 SOSIP Env trimer in complex with PGT121, VRC01 and 3BC315 Fabs 手法: EM (単粒子) / 解像度: 3.16 Å 49239 9nc0 EMDB-49239, PDB-9nc0: AMC008 v4.2 SOSIP Env trimer in complex with b12 Fab 手法: EM (単粒子) / 解像度: 3.88 Å 49240 9nc3 EMDB-49240, PDB-9nc3: AMC008 v4.2 SOSIP Env trimer in complex with b12 and 3BC315 Fabs 手法: EM (単粒子) / 解像度: 3.74 Å 49241 9nc6 EMDB-49241, PDB-9nc6: AMC008 v4.2 SOSIP Env trimer in complex with two 3BC315 Fabs 手法: EM (単粒子) / 解像度: 3.52 Å 49242 9nc8 EMDB-49242, PDB-9nc8: AMC008 v4.2 SOSIP Env trimer in complex with one 3BC315 Fab 手法: EM (単粒子) / 解像度: 3.55 Å 70287 9oaj EMDB-70287, PDB-9oaj: AMC008 v4.2 SOSIP Env trimer in complex with CD4 D1D2 手法: EM (単粒子) / 解像度: 2.91 Å 73342 9yqo EMDB-73342, PDB-9yqo: AMC008 v4.2 SOSIP Env trimer in complex with PGT121 and VRC01 Fabs 手法: EM (単粒子) / 解像度: 3.26 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM / HIV-1 Envelope glycoprotein / broadly neutralizing antibodies / cluster of differentiation 4