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TitleDecreased cytoplasmic crowding via inhibition of ribosome biogenesis can trigger Candida albicans filamentous growth.
Journal, issue, pagesNat Microbiol, Vol. 11, Issue 1, Page 169-179, Year 2026
Publish dateDec 10, 2025
AuthorsAntonio Serrano / Charles Puerner / Louis Chevalier / Emily Plumb / Johannes Elferich / Stephen Diggs / Ludwig Roman Sinn / Nikolaus Grigorieff / Markus Ralser / Morgan Delarue / Martine Bassilana / Robert Alan Arkowitz /
PubMed AbstractThe human fungal pathogen Candida albicans undergoes a morphological transition from a budding yeast to a filamentous form, which is associated with pathogenesis. Various cues mediate this transition ...The human fungal pathogen Candida albicans undergoes a morphological transition from a budding yeast to a filamentous form, which is associated with pathogenesis. Various cues mediate this transition including intracellular reorganization. The cytoplasm is densely packed with proteins including large macromolecular complexes, such as ribosomes, and hence, molecular crowding can impact a range of cellular processes. However, the relationship between cytoplasmic molecular crowding and morphological growth states is unclear. Using a fluorescent microrheological probe and single particle tracking, we observed a striking decrease in molecular crowding during filamentous growth in C. albicans. On the basis of simulations, proteomics and structural data from in situ cryogenic electron microscopy, we show that the reduction in crowding is due to a decrease in ribosome concentration that results in part from an inhibition of ribosome biogenesis, combined with an increase in cytoplasmic volume, leading to a dilution of ribosomes. Filamentation was enhanced in a mutant defective in ribosome biogenesis, while translation was not affected, suggesting that inhibition of ribosome biogenesis is a trigger for C. albicans morphogenesis. Overall, we show that filamentous growth is associated with reduced cytoplasmic crowding via changes in ribosome concentration, suggesting that combination therapies in which ribosome biogenesis is also targeted may be advantageous.
External linksNat Microbiol / PubMed:41372638
MethodsEM (single particle)
Resolution4.0 Å
Structure data

EMDB-72464: Reconstruction of Candida albicans fatty acid synthase from cell slices using 2DTM
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-72488: Reconstruction of Candida albicans ribosomes from cell slices using 2DTM (mRNA decoding Class)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-72489: Reconstruction of Candida albicans ribosomes from cell slices using 2DTM (Peptidyl transfer Class)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-72490: Reconstruction of Candida albicans ribosomes from cell slices using 2DTM (tRNA translocation Class)
Method: EM (single particle) / Resolution: 4.0 Å

Source
  • Candida albicans (yeast)

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