+Search query
-Structure paper
Title | Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. |
---|---|
Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 114, Issue 52, Page 13708-13713, Year 2017 |
Publish date | Dec 26, 2017 |
Authors | Yingyuan Sun / Aaron P Roznowski / Joshua M Tokuda / Thomas Klose / Alexander Mauney / Lois Pollack / Bentley A Fane / Michael G Rossmann / |
PubMed Abstract | Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time- ...Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection states. The ejection is initiated by the loss of the G protein spike that encounters the LPS, followed by conformational changes of two polypeptide loops on the major capsid F proteins. One of these loops mediates viral attachment, and the other participates in making the fivefold channel at the vertex contacting the LPS. |
External links | Proc Natl Acad Sci U S A / PubMed:29229840 / PubMed Central |
Methods | EM (single particle) |
Resolution | 9.9 Å |
Structure data | EMDB-7033: EMDB-8862: |
Source |
|