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-Structure paper
Title | Structure of the intact 14-subunit human cytochrome c oxidase. |
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Journal, issue, pages | Cell Res, Vol. 28, Issue 10, Page 1026-1034, Year 2018 |
Publish date | Jul 20, 2018 |
Authors | Shuai Zong / Meng Wu / Jinke Gu / Tianya Liu / Runyu Guo / Maojun Yang / |
PubMed Abstract | Respiration is one of the most basic features of living organisms, and the electron transport chain complexes are probably the most complicated protein system in mitochondria. Complex-IV is the ...Respiration is one of the most basic features of living organisms, and the electron transport chain complexes are probably the most complicated protein system in mitochondria. Complex-IV is the terminal enzyme of the electron transport chain, existing either as randomly scattered complexes or as a component of supercomplexes. NDUFA4 was previously assumed as a subunit of complex-I, but recent biochemical data suggested it may be a subunit of complex-IV. However, no structural evidence supporting this notion was available till now. Here we obtained the 3.3 Å resolution structure of complex-IV derived from the human supercomplex IIIIIV and assigned the NDUFA4 subunit into complex-IV. Intriguingly, NDUFA4 lies exactly at the dimeric interface observed in previously reported crystal structures of complex-IV homodimer which would preclude complex-IV dimerization. Combining previous structural and biochemical data shown by us and other groups, we propose that the intact complex-IV is a monomer containing 14 subunits. |
External links | Cell Res / PubMed:30030519 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.3 - 3.6 Å |
Structure data | |
Chemicals | ChemComp-CU: ChemComp-MG: ChemComp-HEA: ChemComp-PEE: ChemComp-CDL: ChemComp-ZN: |
Source |
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Keywords | ELECTRON TRANSPORT / cytochrome c oxidase |