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TitleStructural Basis of Heterochromatin Formation by Human HP1.
Journal, issue, pagesMol Cell, Vol. 69, Issue 3, Page 385-397.e8, Year 2018
Publish dateFeb 1, 2018
AuthorsShinichi Machida / Yoshimasa Takizawa / Masakazu Ishimaru / Yukihiko Sugita / Satoshi Sekine / Jun-Ichi Nakayama / Matthias Wolf / Hitoshi Kurumizaka /
PubMed AbstractHeterochromatin plays important roles in transcriptional silencing and genome maintenance by the formation of condensed chromatin structures, which determine the epigenetic status of eukaryotic cells. ...Heterochromatin plays important roles in transcriptional silencing and genome maintenance by the formation of condensed chromatin structures, which determine the epigenetic status of eukaryotic cells. The trimethylation of histone H3 lysine 9 (H3K9me3), a target of heterochromatin protein 1 (HP1), is a hallmark of heterochromatin formation. However, the mechanism by which HP1 folds chromatin-containing H3K9me3 into a higher-order structure has not been elucidated. Here we report the three-dimensional structure of the H3K9me3-containing dinucleosomes complexed with human HP1α, HP1β, and HP1γ, determined by cryogenic electron microscopy with a Volta phase plate. In the structures, two H3K9me3 nucleosomes are bridged by a symmetric HP1 dimer. Surprisingly, the linker DNA between the nucleosomes does not directly interact with HP1, thus allowing nucleosome remodeling by the ATP-utilizing chromatin assembly and remodeling factor (ACF). The structure depicts the fundamental architecture of heterochromatin.
External linksMol Cell / PubMed:29336876
MethodsEM (single particle)
Resolution6.9 - 24.0 Å
Structure data

EMDB-6738:
Human HP1alpha-dinucleosome H3K9Me3 class A
Method: EM (single particle) / Resolution: 11.5 Å

EMDB-6739:
Human HP1alpha-dinucleosome H3K9Me3 class B
Method: EM (single particle) / Resolution: 11.9 Å

EMDB-6740:
Human HP1alpha-dinucleosome H3K9Me3 class C
Method: EM (single particle) / Resolution: 11.5 Å

EMDB-6835:
Mononucleosome from HP1alpha-dinucleosome-H3K9me3
Method: EM (single particle) / Resolution: 6.9 Å

EMDB-6836:
Human HP1beta-dinucleosome H3K9Me3
Method: EM (single particle) / Resolution: 24.0 Å

EMDB-6837:
Human HP1gamma-dinucleosome H3K9Me3
Method: EM (single particle) / Resolution: 24.0 Å

Source
  • Homo sapiens (human)

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