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TitleA binding-block ion selective mechanism revealed by a Na/K selective channel.
Journal, issue, pagesProtein Cell, Vol. 9, Issue 7, Page 629-639, Year 2018
Publish dateSep 18, 2017
AuthorsJie Yu / Bing Zhang / Yixiao Zhang / Cong-Qiao Xu / Wei Zhuo / Jingpeng Ge / Jun Li / Ning Gao / Yang Li / Maojun Yang /
PubMed AbstractMechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of ...Mechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the YnaI channel as the Na/K cation-selective MS channel and solved its structure at 3.8 Å by cryo-EM single-particle method. YnaI exhibits low conductance among the family of MS channels in E. coli, and shares a similar overall heptamer structure fold with previously studied MscS channels. By combining structural based mutagenesis, quantum mechanical and electrophysiological characterizations, we revealed that ion selective filter formed by seven hydrophobic methionine (YnaI) in the transmembrane pore determined ion selectivity, and both ion selectivity and gating of YnaI channel were affected by accompanying anions in solution. Further quantum simulation and functional validation support that the distinct binding energies with various anions to YnaI facilitate Na/K pass through, which was defined as binding-block mechanism. Our structural and functional studies provided a new perspective for understanding the mechanism of how MS channels select ions driven by mechanical force.
External linksProtein Cell / PubMed:28921397 / PubMed Central
MethodsEM (single particle)
Resolution3.8 Å
Structure data

6805

5y4o

EMDB-6805, PDB-5y4o:
Cryo-EM structure of MscS channel, YnaI
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • escherichia coli o157:h7 (bacteria)
KeywordsMEMBRANE PROTEIN / cryo-EM / MscS / Na/K selective channel

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