Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Flagellum couples cell shape to motility in . |
|---|---|
| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 115, Issue 26, Page E5916-E5925, Year 2018 |
| Publish date | Jun 26, 2018 |
 Authors | Stella Y Sun / Jason T Kaelber / Muyuan Chen / Xiaoduo Dong / Yasaman Nematbakhsh / Jian Shi / Matthew Dougherty / Chwee Teck Lim / Michael F Schmid / Wah Chiu / Cynthia Y He /   |
| PubMed Abstract | In the unicellular parasite the causative agent of human African sleeping sickness, complex swimming behavior is driven by a flagellum laterally attached to the long and slender cell body. Using ...In the unicellular parasite the causative agent of human African sleeping sickness, complex swimming behavior is driven by a flagellum laterally attached to the long and slender cell body. Using microfluidic assays, we demonstrated that can penetrate through an orifice smaller than its maximum diameter. Efficient motility and penetration depend on active flagellar beating. To understand how active beating of the flagellum affects the cell body, we genetically engineered to produce anucleate cytoplasts (zoids and minis) with different flagellar attachment configurations and different swimming behaviors. We used cryo-electron tomography (cryo-ET) to visualize zoids and minis vitrified in different motility states. We showed that flagellar wave patterns reflective of their motility states are coupled to cytoskeleton deformation. Based on these observations, we propose a mechanism for how flagellum beating can deform the cell body via a flexible connection between the flagellar axoneme and the cell body. This mechanism may be critical for to disseminate in its host through size-limiting barriers. |
 External links | Proc Natl Acad Sci U S A / PubMed:29891682 / PubMed Central |
| Methods | EM (tomography) |
| Structure data |  EMDB-6748:  EMDB-6749:  EMDB-6750: |
| Source |
|
Trypanosoma brucei (eukaryote)