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TitleStructure and assembly of Borna disease virus 1 nucleoprotein-RNA complexes.
Journal, issue, pagesSci Adv, Vol. 12, Issue 15, Page eaeb0835, Year 2026
Publish dateApr 10, 2026
AuthorsYukihiko Sugita / Yuya Hirai / Shinya H Goto / Takuro Fujiwara / Keizo Tomonaga / Takeshi Noda / Masayuki Horie /
PubMed AbstractStructures of nucleoprotein (N)-RNA complexes of the , a virus family in the order , have not been reported. Here, using cryo-electron microscopy (cryo-EM), we report high-resolution structures of ...Structures of nucleoprotein (N)-RNA complexes of the , a virus family in the order , have not been reported. Here, using cryo-electron microscopy (cryo-EM), we report high-resolution structures of Borna disease virus 1 (BoDV-1) N-RNA complex assemblies, including a dominant hexameric ring-like complex and less populated heptameric and octameric forms, the first RNA-bound N structures reported from this family. These structures reveal key features of N-RNA engagement and a BoDV-1-specific stoichiometry of eight nucleotides per N, providing a framework for comparison with related negative-strand RNA viruses. In addition to these RNA-bound complexes, we identified multiple RNA-free oligomers, indicating substantial conformational flexibility of N. Mutational analyses identified residues essential for nucleocapsid formation and RNA synthesis. Cryo-EM of mutant complexes captured RNA-free assemblies, suggesting that initial N oligomerization precedes RNA binding. These findings clarify the structural organization of the N-RNA complex and suggest how oligomeric plasticity contributes to nucleocapsid assembly.
External linksSci Adv / PubMed:41961918 / PubMed Central
MethodsEM (single particle)
Resolution3.57 - 7.83 Å
Structure data

67470

21al

EMDB-67470, PDB-21al:
Tetrameric complex of the Borna disease virus 1 nucleoprotein (mutant Arg341Ala)
Method: EM (single particle) / Resolution: 3.57 Å

EMDB-67471: Asymmetric tetrameric complex of the Borna disease virus 1 nucleoprotein (mutant Arg341Ala)
Method: EM (single particle) / Resolution: 7.01 Å

EMDB-67472: Tetrameric complexes of the Borna disease virus 1 nucleoprotein (mutant Arg341Ala) in an antiparallel configuration
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-67473: Hexameric complex of the Borna disease virus 1 nucleoprotein bound to RNA (mutant Arg341Ala)
Method: EM (single particle) / Resolution: 7.83 Å

EMDB-67474: Heptameric complex of the Borna disease virus 1 nucleoprotein bound to RNA (mutant Arg341Ala)
Method: EM (single particle) / Resolution: 7.76 Å

Source
  • borna disease virus 1
KeywordsVIRAL PROTEIN / nucleoprotein / complex

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