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- EMDB-61925: Triangular complex of Borna disease virus 1 nucleoprotein with 12... -

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Basic information

Entry
Database: EMDB / ID: EMD-61925
TitleTriangular complex of Borna disease virus 1 nucleoprotein with 12 subunits
Map dataprimary map
Sample
  • Complex: Nucleoprotein complexes of the Borna disease virus 1
    • Protein or peptide: Nucleoprotein
Keywordsnucleoprotein / complex / VIRAL PROTEIN
Biological speciesBorna disease virus 1
Methodsingle particle reconstruction / cryo EM / Resolution: 7.93 Å
AuthorsSugita Y / Hirai Y / Horie M
Funding support Japan, 2 items
OrganizationGrant numberCountry
Japan Science and Technology21460759 Japan
Ministry of Education, Culture, Sports, Science and Technology (Japan)24K02284 Japan
CitationJournal: To Be Published
Title: Tetrameric nucleoprotein complex of the Borna disease virus 1 nucleoprotein (3D class 1)
Authors: Sugita Y / Hirai Y / Horie M
History
DepositionOct 14, 2024-
Header (metadata) releaseMar 11, 2026-
Map releaseMar 11, 2026-
UpdateMar 11, 2026-
Current statusMar 11, 2026Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_61925.png

Downloads & links

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Map

FileDownload / File: emd_61925.map.gz / Format: CCP4 / Size: 15 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationprimary map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.19 Å/pix.
x 158 pix.
= 188.652 Å		1.19 Å/pix.
x 158 pix.
= 188.652 Å		1.19 Å/pix.
x 158 pix.
= 188.652 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.194 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.12673464 - 0.35547668
Average (Standard dev.)0.0017851647 (±0.037767515)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions158158158
Spacing158158158
CellA=B=C: 188.65201 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_61925_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map 1

Fileemd_61925_half_map_1.map
Annotationhalf map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map 2

Fileemd_61925_half_map_2.map
Annotationhalf map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Nucleoprotein complexes of the Borna disease virus 1

EntireName: Nucleoprotein complexes of the Borna disease virus 1
Components
  • Complex: Nucleoprotein complexes of the Borna disease virus 1
    • Protein or peptide: Nucleoprotein

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Supramolecule #1: Nucleoprotein complexes of the Borna disease virus 1

SupramoleculeName: Nucleoprotein complexes of the Borna disease virus 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Borna disease virus 1

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Macromolecule #1: Nucleoprotein

MacromoleculeName: Nucleoprotein / type: protein_or_peptide / ID: 1
Details: Full-length nucleoprotein of the Borna disease virus 1 strain He/80 with N-terminal hexahistidine tag
Enantiomer: LEVO
Source (natural)Organism: Borna disease virus 1
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MGSSHHHHHH SSGLVPRGSH MLE MPPKRR LVDDADAMED QDLYEPPASL PKLPGKFLQY TVGGSDPHPG IGHEKDIRQN AVALLDQSRR DMFHTVTP S LVFLCLLIPG LHAAFVHGGV PRESYLSTPV TRGEQTVVKT AKFYGEKTTQ RDLTELEISS IFSHCCSLLI ...String:
MGSSHHHHHH SSGLVPRGSH MLE MPPKRR LVDDADAMED QDLYEPPASL PKLPGKFLQY TVGGSDPHPG IGHEKDIRQN AVALLDQSRR DMFHTVTP S LVFLCLLIPG LHAAFVHGGV PRESYLSTPV TRGEQTVVKT AKFYGEKTTQ RDLTELEISS IFSHCCSLLI GVVIGSSSK IKAGAEQIKK RFKTMMAALN RPSHGETATL LQMFNPHEAI DWINGQPWVG SFVLSLLTTD FESPGKEFMD QIKLVASYAQ MTTYTTIKE YLAECMDATL TIPVVAYEIR DFLEVSAKLK EEHADLFPFL GAIRHPDAIK LAPRSFPNLA SAAFYWSKKE N PTMAGYRA STIQPGASVK ETQLARYRRR EISRGEDGAE LSGEISAIMR MIGVTGLN

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.056 mm / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 10212934
CTF correctionSoftware - Name: cryoSPARC / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 7.93 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 6085
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC
Final 3D classificationNumber classes: 3 / Avg.num./class: 161319 / Software - Name: cryoSPARC
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

1n93


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: RECIPROCAL / Protocol: AB INITIO MODEL

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