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TitleCryo-EM structures of the ATP-bound Vps4 hexamer and its complex with Vta1 at near-atomic resolution.
Journal, issue, pagesNat Commun, Vol. 8, Page 16064, Year 2017
Publish dateJul 17, 2017
AuthorsShan Sun / Lin Li / Fan Yang / Xiaojing Wang / Fenghui Fan / Mengyi Yang / Chunlai Chen / Xueming Li / Hong-Wei Wang / Sen-Fang Sui /
PubMed AbstractThe cellular ESCRT-III (endosomal sorting complex required for transport-III) and Vps4 (vacuolar protein sorting 4) comprise a common machinery that mediates a variety of membrane remodelling events. ...The cellular ESCRT-III (endosomal sorting complex required for transport-III) and Vps4 (vacuolar protein sorting 4) comprise a common machinery that mediates a variety of membrane remodelling events. Vps4 is essential for the machinery function by using the energy from ATP hydrolysis to disassemble the ESCRT-III polymer into individual proteins. Here, we report the structures of the ATP-bound Vps4 hexamer and its complex with the cofactor Vta1 (vps twenty associated 1) at resolutions of 3.9 and 4.2 Å, respectively, determined by electron cryo-microscopy. Six Vps4 subunits in both assemblies exhibit a spiral-shaped ring-like arrangement. Locating at the periphery of the hexameric ring, Vta1 dimer bridges two adjacent Vps4 subunits by two different interaction modes to promote the formation of the active Vps4 hexamer during ESCRT-III filament disassembly. The structural findings, together with the structure-guided biochemical and single-molecule analyses, provide important insights into the process of the ESCRT-III polymer disassembly by Vps4.
External linksNat Commun / PubMed:28714467 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.25 Å
Structure data

6733

5xmi

EMDB-6733, PDB-5xmi:
Cryo-EM Structure of the ATP-bound VPS4 mutant-E233Q hexamer (masked)
Method: EM (single particle) / Resolution: 3.9 Å

6734

5xmk

EMDB-6734, PDB-5xmk:
Cryo-EM structure of the ATP-bound Vps4 mutant-E233Q complex with Vta1 (masked)
Method: EM (single particle) / Resolution: 4.18 Å

EMDB-6735:
Cryo-EM structure of the ATP-bound VPS4 mutant-E233Q hexamer (unmasked)
Method: EM (single particle) / Resolution: 4.08 Å

EMDB-6736:
Cryo-EM structure of ATP-bound Vps4 mutant-E233Q complex with Vta1 (unmasked)
Method: EM (single particle) / Resolution: 4.25 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • Saccharomyces cerevisiae S288c (yeast)
KeywordsPROTEIN TRANSPORT / ATPase / EESCRTIII / Vps4 / ESCRTIII / Vta1

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