Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural atlas of the intact jumbo phage phiKZ.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	Apr 13, 2026
Authors	Hao Xiao / Zeng Peng / Junquan Zhou / Yuan Chen / Yuning Peng / Yixiong Tang / Tao Li / Wenyuan Chen / Sheng-You Huang / Lingpeng Cheng / Hongrong Liu /
PubMed Abstract	Jumbo phage phiKZ, a key model for studying phage nucleus formation and bacterial defense mechanisms, possesses a highly complex tail machine that is essential for infection. Here, we present the ...Jumbo phage phiKZ, a key model for studying phage nucleus formation and bacterial defense mechanisms, possesses a highly complex tail machine that is essential for infection. Here, we present the structural atlas of the intact jumbo phage phiKZ by cryo-EM, thereby identifying 40 constituent proteins and unveiling its modular architecture. The virion, with a length of approximately 360 nm, is comprised of an icosahedral capsid of 2520 polypeptide chains from 11 proteins, and a massive tail machine of over 900 polypeptide chains from 29 proteins. The tail features a unique, multi-layered neck and a highly elaborate baseplate. The neck is reinforced by whisker-like proteins and anchors the contractile tail, which terminates in the baseplate. The baseplate is constituted by a central hub, an inner periphery of interlocking wedge heterotrimers and hexagonal rings, and an outer periphery with a striking hexagonal star configuration. This intricate peripheral region of the baseplate serves as an extended platform for twelve peripheral fibers, which mediate host cell adsorption. Our findings provide a structural framework for understanding jumbo phage assembly and infection, thus contributing to the foundation for future functional studies and rational engineering of these phages for potential therapeutic applications.
External links	Nat Commun / PubMed:41974715
Methods	EM (single particle)
Resolution	3.15 - 7.61 Å
Structure data	67095 9xpe EMDB-67095, PDB-9xpe: Structure of the Portal and Adaptor Proteins of the Phage Phikz Method: EM (single particle) / Resolution: 3.37 Å 67096 9xpf EMDB-67096, PDB-9xpf: The structure of sheath and tube proteins of phage Phikz Method: EM (single particle) / Resolution: 3.15 Å 67097 9xpg EMDB-67097, PDB-9xpg: The structure of gp139 protein of phage phikz Method: EM (single particle) / Resolution: 4.14 Å 67098 9xph EMDB-67098, PDB-9xph: The structure of baseplate central region of phage phikz Method: EM (single particle) / Resolution: 4.02 Å 67102 9xps EMDB-67102, PDB-9xps: The neck structure of the Phage Phikz Method: EM (single particle) / Resolution: 3.52 Å 67111 9xqd EMDB-67111, PDB-9xqd: The structure of outer peripheral region in the phage phiKZ baseplate complex Method: EM (single particle) / Resolution: 4.53 Å 67121 9xqs EMDB-67121, PDB-9xqs: Structure of the inner peripheral region in the phage phiKZ baseplate complex Method: EM (single particle) / Resolution: 3.65 Å EMDB-67123: Map of the unit1 in the phage phiKZ baseplate complex Method: EM (single particle) / Resolution: 4.39 Å EMDB-67124: Map of the unit2 in the phage phiKZ baseplate complex Method: EM (single particle) / Resolution: 4.55 Å EMDB-67125: The overall tail of phage PhiKZ Method: EM (single particle) / Resolution: 7.61 Å
Source	pseudomonas phage phikz (virus)
Keywords	VIRAL PROTEIN / portal / adaptor / sheath / tube / baseplate / inner peripheral / spike / neck / outer peripheral