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TitleStructural insights into σ28-dependent transcription initiation and its regulation by anti-sigma factor in Pseudomonas aeruginosa.
Journal, issue, pagesNucleic Acids Res, Vol. 54, Issue 1, Year 2026
Publish dateJan 5, 2026
Authors Sheenu / Vineet Kumar / Pankaj Kumar Sahoo / Eaazhisai Kandiah / Deepti Jain /
PubMed AbstractLate flagellar genes in Pseudomonas aeruginosa are transcribed by the group 3 sigma factor, FliA (σ28). σ28 drives the expression of flagellin, which assembles into the flagellar filament in this ...Late flagellar genes in Pseudomonas aeruginosa are transcribed by the group 3 sigma factor, FliA (σ28). σ28 drives the expression of flagellin, which assembles into the flagellar filament in this monoflagellated bacterium. This function is suppressed by the anti-sigma factor FlgM. Here, we present the 1.95Å resolution crystal structure of σ28-FlgM complex, along with a 3.4Å structure of σ28RNAP open promoter complex determined using single particle cryo-electron microscopy from P. aeruginosa. The σ28 adopts a compact conformation upon binding to the anti-sigma factor FlgM, which contacts all three domains of the sigma factor. This conformation is neither conducive to interactions with RNA polymerase nor the promoter DNA. The cryo-EM structure reveals base-specific interactions of σ28 domain 4 (σ4) with -35 element, flipping of -11 base of the template strand, novel interactions of template strand with domain 2 (σ2) and 3 (σ3), and partial insertion of sigma finger into the active site cleft, offering unique features of group 3 sigma interactions with promoter DNA. Perturbation of key residues affects transcription in vitro and flagellar phenotypes as well as bacterial motility in vivo. Analysis of the structural data presented here reveals new insights into transcription regulation of late flagellar genes.
External linksNucleic Acids Res / PubMed:41521663 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.95 - 3.4 Å
Structure data

66199

9wsm

EMDB-66199, PDB-9wsm:
Cryo-EM structure of Sigma28-RNAP open promoter complex from Pseudomonas aeruginosa
Method: EM (single particle) / Resolution: 3.4 Å

PDB-9wsf:
Crystal structure of Sigma28/FlgM complex from Pseudomonas aeruginosa at 1.95 Angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsTRANSCRIPTION / P. aeruginosa / FliA/FlgM / Flagella / Sigma 28 / RNAP / fliC

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