Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural insights into the progressive recovery of α7 nicotinic acetylcholine receptor from nicotine-induced desensitization.
Journal, issue, pages	Sci Adv, Vol. 11, Issue 41, Page eadx4432, Year 2025
Publish date	Oct 10, 2025
Authors	Sanling Liu / Haopeng Chen / Xiaohong Zhu / Fei Ye / Yue Zhao / Junlin Qin / Yining Zheng / Xudong Wang / Longhua Zhang / Huan Chen / Xin Li / Wenjun Mu / Yaning Fu / Cheng Luo / Hongwei Hou / Chen Bai / Lei Liu / Changlin Tian /
PubMed Abstract	Nicotine is the predominant alkaloid in tobacco leaves and affects the human nervous system by interacting with nicotinic acetylcholine receptors (nAChRs). Chronic nicotine exposure leads to nAChR ...Nicotine is the predominant alkaloid in tobacco leaves and affects the human nervous system by interacting with nicotinic acetylcholine receptors (nAChRs). Chronic nicotine exposure leads to nAChR desensitization, while nicotine withdrawal leads to nAChR recovery. However, detailed molecular mechanisms underlying nicotine-induced nAChR desensitization and its recovery remain elusive. Here, we present cryo-EM structures of the α7 nAChR in complex with nicotine in both an open state and multiple desensitized states. Comparative analyses reveal progressive conformational changes during recovery from nicotine-induced desensitization and show that asymmetric nicotine binding disrupts the symmetry of the channel pore at the 16' and 17' sites. Integrating these findings with patch-clamp recordings and computational simulations, we identify an agonist-free structure that represents an atypical desensitized state closely resembling the resting conformation of α7 nAChR. These detailed mechanistic studies enhance our understanding of nicotine's effects on α7 nAChRs.
External links	Sci Adv / PubMed:41071887 / PubMed Central
Methods	EM (single particle)
Resolution	2.61 - 3.25 Å
Structure data	63084 9lh5 EMDB-63084, PDB-9lh5: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (open state) Method: EM (single particle) / Resolution: 2.61 Å 63086 9lh6 EMDB-63086, PDB-9lh6: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (five-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 2.69 Å 63089 9lh8 EMDB-63089, PDB-9lh8: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (four-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 2.74 Å 63090 9lh9 EMDB-63090, PDB-9lh9: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (ortho-three-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 2.98 Å 63091 9lha EMDB-63091, PDB-9lha: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (meta-three-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 2.96 Å 63092 9lhb EMDB-63092, PDB-9lhb: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (ortho-two-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 3.11 Å 63093 9lhc EMDB-63093, PDB-9lhc: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (meta-two-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 3.17 Å 63094 9lhd EMDB-63094, PDB-9lhd: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (one-nicotine-bound desensitized state) Method: EM (single particle) / Resolution: 3.24 Å 63095 9lhe EMDB-63095, PDB-9lhe: human alpha 7 nicotinic acetylcholine receptor in complex with L-nicotine (nicotine-free desensitized state) Method: EM (single particle) / Resolution: 3.25 Å 65096 9viq EMDB-65096, PDB-9viq: human alpha 7 nicotinic acetylcholine receptor L277A mutant in complex with L-nicotine (desensitized state) Method: EM (single particle) / Resolution: 3.24 Å 65097 9vix EMDB-65097, PDB-9vix: human alpha 7 nicotinic acetylcholine receptor L278A mutant in complex with L-nicotine (desensitized state) Method: EM (single particle) / Resolution: 3.06 Å
Chemicals	ChemComp-NCT: (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-HOH: WATER
Source	homo sapiens (human)
Keywords	MEMBRANE PROTEIN / Ligand-gated ion channel / Nicotine / Desensitization / Recovery / Open pore / Closed pore / mutant