EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into higher-order natural RNA-only multimers.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 10, Page 2012-2021, Year 2025
掲載日	2025年8月6日
著者	Shengchun Zhang / Ran Yi / Linfeng An / Ji Liu / Xuebiao Yao / Shanshan Li / Kaiming Zhang /
PubMed 要旨	RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in ...RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in synthetic RNA nanomaterials, naturally occurring RNA-only complexes have primarily been observed as monomers or dimers, leaving higher-order assemblies largely unexplored. ROOL (rumen-originating, ornate, large) and GOLLD (giant, ornate, lake- and Lactobacillales-derived) RNAs are conserved non-coding RNAs with complex secondary structures, but their high-resolution architectures remain unknown. Here, we determine the cryo-electron microscopy structures of UCC118-Rool RNA, Sag-Golld RNA and Env38-Golld RNA at 1.96-2.98 Å resolution, revealing their distinct hexameric, decameric and tetradecameric assemblies. These higher-order architectures are stabilized by an array of tertiary motifs such as kissing loops and tetraloop-receptor motifs, underscoring the conserved principles of RNA self-assembly. By elucidating the molecular details of these higher-order RNA-only assemblies, this study expands our understanding of RNA-based architectures and broadens the scope of RNA structural biology.
リンク	Nat Struct Mol Biol / PubMed:40770114
手法	EM (単粒子)
解像度	1.96 - 3.83 Å
構造データ	63014 9le3 EMDB-63014, PDB-9le3: Consensus map of UCC118 Rool RNA 手法: EM (単粒子) / 解像度: 2.75 Å 63015 9le5 EMDB-63015, PDB-9le5: Focused asymmetric unit of UCC118 Rool RNA 手法: EM (単粒子) / 解像度: 2.58 Å 63016 9le6 EMDB-63016, PDB-9le6: Composite map of UCC118 Rool RNA 手法: EM (単粒子) / 解像度: 2.58 Å EMDB-63019: Consensus map of Sag-18RS21 Golld RNA 手法: EM (単粒子) / 解像度: 3.3 Å 63022 9lec EMDB-63022, PDB-9lec: Focused asymmetric unit of Sag-18RS21 Golld RNA 手法: EM (単粒子) / 解像度: 2.51 Å 63023 9lee EMDB-63023, PDB-9lee: Composite map of Sag-18RS21 Golld RNA 手法: EM (単粒子) / 解像度: 2.51 Å EMDB-63026: Consensus map of Sag-18RS21-tRNASer Golld RNA 手法: EM (単粒子) / 解像度: 3.83 Å 63028 9lel EMDB-63028, PDB-9lel: Focused asymmetric unit of Sag-18RS21-tRNASer Golld RNA 手法: EM (単粒子) / 解像度: 2.98 Å 63029 9lem EMDB-63029, PDB-9lem: Composite map of Sag-18RS21-tRNASer Golld RNA 手法: EM (単粒子) / 解像度: 2.98 Å EMDB-63099: Consensus map of Env38-Golld RNA 手法: EM (単粒子) / 解像度: 3.52 Å 63100 9lhk EMDB-63100, PDB-9lhk: Focused asymmetric unit of Env38-Golld RNA 手法: EM (単粒子) / 解像度: 2.93 Å 63101 9lhl EMDB-63101, PDB-9lhl: Composite map of Env38-Golld RNA 手法: EM (単粒子) / 解像度: 2.93 Å 63678 9m78 EMDB-63678, PDB-9m78: Consensus map of UCC118 Rool RNA at 2.26-angstrom resolution 手法: EM (単粒子) / 解像度: 2.26 Å 63679 9m79 EMDB-63679, PDB-9m79: Focused asymmetric unit of UCC118 Rool RNA at 1.96-angstrom resolution 手法: EM (単粒子) / 解像度: 1.96 Å
由来	ligilactobacillus salivarius ucc118 (バクテリア) streptococcus agalactiae 18rs21 (バクテリア) uncultured caudovirales phage (ファージ)
キーワード	RNA / Natural RNA nanocages; large RNA; cryo-EM; Rool / Natural RNA nanocages; large RNA; cryo-EM; Golld / Natural RNA nanocages; large RNA; cryo-EM; Golld RNA / Natural RNA-Only Multimer / cryo-EM / Oligomer / Rool.