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Structure paper

TitleMechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 10291, Year 2025
Publish dateNov 21, 2025
AuthorsAlexandra I Vancea / Brandon Huntington / Wieland Steinchen / Christos G Savva / Umar F Shahul Hameed / Stefan T Arold /
PubMed AbstractStrigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone ...Strigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone perception by receptors initiates the degradation of transcriptional repressors via E3 ubiquitin ligases, but the mechanistic link between hormone binding and substrate ubiquitination has remained unclear. We determine cryogenic electron microscopy structures of the receptor-ligase-substrate complex, composed of Arabidopsis ASK1 and substrate, and Striga F-box and receptor proteins. Strigolactone hydrolysis by the receptor, which covalently retains the D-ring, is a prerequisite for complex formation. The substrate engages the complex through two domains, forming a dynamic interface that stabilises the receptor-ligase assembly and repositions the ASK1, suggesting a mechanism for efficient ubiquitination. Here, we show how dynamic, multivalent interactions within the receptor-ligase-substrate complex translate hormone perception into targeted protein degradation, providing insight into how plants integrate hormonal signals into developmental decisions.
External linksNat Commun / PubMed:41271672 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 4.3 Å
Structure data

62397

9kkx

EMDB-62397, PDB-9kkx:
Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 1) with covalently bound D-ring
Method: EM (single particle) / Resolution: 2.8 Å

62401

9kld

EMDB-62401, PDB-9kld:
Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 2) with covalently bound D-ring
Method: EM (single particle) / Resolution: 2.9 Å

62407

9klk

EMDB-62407, PDB-9klk:
Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 4) with covalently bound D-ring
Method: EM (single particle) / Resolution: 2.9 Å

62408

9kll

EMDB-62408, PDB-9kll:
Striga MAX2-ASK1 complex
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-62414: Striga MAX2-ASK1 2:2 dimer
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-62415: Rice D3-ASK1 complex in presence of ShHTL7 with GR24
Method: EM (single particle) / Resolution: 4.3 Å

62417

9klv

EMDB-62417, PDB-9klv:
Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 3) with covalently bound D-ring
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-GR2:
(3E,3aR,8bS)-3-({[(2R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yl]oxy}methylidene)-3,3a,4,8b-tetrahydro-2H-indeno[1,2-b]furan-2-one

Source
  • arabidopsis thaliana (thale cress)
  • striga hermonthica (purple witchweed)
  • Oryza sativa (Asian cultivated rice)
KeywordsSIGNALING PROTEIN / Strigolactone / SCF / Ubiquitination

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