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TitleMolecular mechanism of phosphate import by the bacterial PstSCAB transporter.
Journal, issue, pagesNat Commun, Year 2026
Publish dateFeb 3, 2026
AuthorsHu Xiao / Shanqin Li / Ruxi Qi / Yuxiang Hu / Xiaozi Jiang / Jing Luo / Jing Wu / Lei Zhang / Shuman Xu / Defen Lu / Xinwang Yang / Qingfeng Chen / Sheng Liu /
PubMed AbstractInorganic phosphate (Pi) is essential for all living organisms. PstSCAB, a bacterial high-affinity ABC transporter, imports Pi under limiting conditions via five subunits: PstA and PstC forming the ...Inorganic phosphate (Pi) is essential for all living organisms. PstSCAB, a bacterial high-affinity ABC transporter, imports Pi under limiting conditions via five subunits: PstA and PstC forming the transmembrane domain (TMD), periplasmic PstS that switches between free and TMD-docked forms for Pi capture and delivery, and two cytosolic PstB subunits for ATP binding and hydrolysis. Its malfunction affects the virulence of pathogenic bacteria, making it pharmaceutically attractive. However, complete structural pictures of PstSCAB in different states remain lacking. Here, we determine cryo-EM structures of PstSCAB in resting, pretranslocation, and catalytic intermediate states, which reveal that conformational changes in PstS and ATP binding/unbinding in PstB collectively induce rigid-body movements of TMD, generating inward- or outward-facing conformations. In TMD, Pi specificity is determined by positively charged Arg220 (PstA) and Arg237 (PstC). This study advances understanding of bacterial Pi import and supports drug development targeting PstSCAB.
External linksNat Commun / PubMed:41634007
MethodsEM (single particle)
Resolution2.7 - 3.0 Å
Structure data

62027

9k3s

EMDB-62027, PDB-9k3s:
Cryo-EM structure of E coli pstSCAB in the catalytic intermediate state
Method: EM (single particle) / Resolution: 2.7 Å

62031

9k3x

EMDB-62031, PDB-9k3x:
Cryo-EM structure of E coli pstSCAB in the pretranslocation state
Method: EM (single particle) / Resolution: 2.9 Å

62032

9k3y

EMDB-62032, PDB-9k3y:
Cryo-EM structure of E coli pstSCAB in the resting state
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • Escherichia coli (strain K12) (bacteria)
  • escherichia coli k-12 (bacteria)
KeywordsMETAL TRANSPORT / ABC transporter / Phosphate transport / pstSCAB complex

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