EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plants.
ジャーナル・号・ページ	EMBO J, Vol. 45, Issue 9, Page 3192-3205, Year 2026
掲載日	2026年4月2日
著者	Guohui Xie / Xuan Du / Yifang Tan / Yuxing Zhou / Cheng Chi / Sixian Zhou / Colette L Picard / Songge Chai / Lei Wu / Danling Zhu / Jun Zhao / Yan Xue / Sisi Li / Steven E Jacobsen / Zhe Wu / Jiamu Du /
PubMed 要旨	DNA methylation plays critical roles in eukaryotic gene silencing, genome imprinting, viral defense, and suppression of transposable elements. In plants, RNA Polymerase V (Pol V)-generated non-coding ...DNA methylation plays critical roles in eukaryotic gene silencing, genome imprinting, viral defense, and suppression of transposable elements. In plants, RNA Polymerase V (Pol V)-generated non-coding RNA guides DNA methylation through the RNA-directed DNA methylation (RdDM) pathway; however, how these RNAs are selected is unknown. Here, we show that the 3'-ends of Pol V transcripts are enriched at A-rich template DNA (A-rich-DNA). Arabidopsis RdDM regions possess AT-rich boundaries genome-wide, suggesting that Pol V likely terminates at A-rich-DNA, which subsequently defines the DNA methylation landscape in plants. A-rich-DNA successfully stops Pol V transcription in vitro. Structural snapshots of Pol V transcribing A-rich-DNA show that accumulation of unstable rU:dA pairs in the RNA-DNA hybrid promotes transcription bubble collapse and spontaneous transcription termination. These findings identify an intrinsic Pol V termination signal that shapes genomic DNA methylation patterning in plants and reveals a common mechanism for spontaneous transcription termination.
リンク	EMBO J / PubMed:41928006 / PubMed Central
手法	EM (単粒子)
解像度	2.96 - 4.06 Å
構造データ	61961 9k11 EMDB-61961, PDB-9k11: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 0U scaffold at 2.96 Angstrom 手法: EM (単粒子) / 解像度: 2.96 Å 61962 9k12 EMDB-61962, PDB-9k12: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 1U sacffold at 3.5 Angstrom 手法: EM (単粒子) / 解像度: 3.46 Å 61963 9k13 EMDB-61963, PDB-9k13: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 2U sacffold at 3.04 Angstrom 手法: EM (単粒子) / 解像度: 3.04 Å 61964 9k14 EMDB-61964, PDB-9k14: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 3U sacffold at 3.8 Angstrom 手法: EM (単粒子) / 解像度: 3.8 Å 61965 9k15 EMDB-61965, PDB-9k15: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 4U sacffold at 3.32 Angstrom 手法: EM (単粒子) / 解像度: 3.32 Å 61966 9k16 EMDB-61966, PDB-9k16: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 5U sacffold at 3.19 Angstrom 手法: EM (単粒子) / 解像度: 3.19 Å 61967 9k17 EMDB-61967, PDB-9k17: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 6U sacffold at 3.04 Angstrom 手法: EM (単粒子) / 解像度: 3.04 Å 61968 9k18 EMDB-61968, PDB-9k18: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 7U sacffold at 3.42 Angstrom 手法: EM (単粒子) / 解像度: 3.42 Å 61969 9k19 EMDB-61969, PDB-9k19: A cryo-EM structure of B. oleracea RNA polymerase V in complex with 8U sacffold at 4.06 Angstrom 手法: EM (単粒子) / 解像度: 4.06 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry
由来	brassica oleracea (キャベツ)
キーワード	TRANSCRIPTION / DNA-dependent RNA polymerase V / plant