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-Structure paper
| タイトル | Structure and assembly of Borna disease virus 1 nucleoprotein-RNA complexes. |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 12, Issue 15, Page eaeb0835, Year 2026 |
| 掲載日 | 2026年4月10日 |
 著者 | Yukihiko Sugita / Yuya Hirai / Shinya H Goto / Takuro Fujiwara / Keizo Tomonaga / Takeshi Noda / Masayuki Horie /  |
| PubMed 要旨 | Structures of nucleoprotein (N)-RNA complexes of the , a virus family in the order , have not been reported. Here, using cryo-electron microscopy (cryo-EM), we report high-resolution structures of ...Structures of nucleoprotein (N)-RNA complexes of the , a virus family in the order , have not been reported. Here, using cryo-electron microscopy (cryo-EM), we report high-resolution structures of Borna disease virus 1 (BoDV-1) N-RNA complex assemblies, including a dominant hexameric ring-like complex and less populated heptameric and octameric forms, the first RNA-bound N structures reported from this family. These structures reveal key features of N-RNA engagement and a BoDV-1-specific stoichiometry of eight nucleotides per N, providing a framework for comparison with related negative-strand RNA viruses. In addition to these RNA-bound complexes, we identified multiple RNA-free oligomers, indicating substantial conformational flexibility of N. Mutational analyses identified residues essential for nucleocapsid formation and RNA synthesis. Cryo-EM of mutant complexes captured RNA-free assemblies, suggesting that initial N oligomerization precedes RNA binding. These findings clarify the structural organization of the N-RNA complex and suggest how oligomeric plasticity contributes to nucleocapsid assembly. |
 リンク | Sci Adv / PubMed:41961918 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.78 - 9.12 Å |
| 構造データ | EMDB-61914, PDB-9jzi: EMDB-61915, PDB-9jzj: EMDB-61916, PDB-9jzk: EMDB-61917, PDB-9jzl:  EMDB-61918: Heptameric ring-like complex of the Borna disease virus 1 nucleoprotein bound to RNA  EMDB-61919: Octameric ring-like complex of the Borna disease virus 1 nucleoprotein bound to RNA  EMDB-61920: Hexameric flat ring-like complex of the Borna disease virus 1 nucleoprotein  EMDB-61921: Hexameric oval ring-like complex of Borna disease virus 1 nucleoprotein with central extra density  EMDB-61922: Hexameric oval ring-like complex of Borna disease virus 1 nucleoprotein  EMDB-61923: S-shaped octameric complex of the Borna disease virus 1 nucleoprotein  EMDB-61924: S-shaped nonameric complex of Borna disease virus 1 nucleoprotein  EMDB-61925: Triangular complex of Borna disease virus 1 nucleoprotein with 12 subunits  EMDB-61926: Triangular complex of the Borna disease virus 1 nucleoprotein with 13 subunits  EMDB-61927: Tetrameric complexes of the Borna disease virus 1 nucleoprotein arranged in a parallel configuration  EMDB-61928: Tetrameric complexes of the Borna disease virus 1 nucleoprotein are arranged in an antiparallel configuration EMDB-61929, PDB-9jzn:  EMDB-61930: Pentameric complex of Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61931: Hexameric ring-like complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61932: Heptameric ring-like complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61933: Hexameric flat ring-like complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61934: Hexameric oval ring-like complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61935: Heptameric flat ring-like complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61936: S-shaped octameric complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61937: Triangular hexameric complex of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala)  EMDB-61938: Tetrameric complexes of the Borna disease virus 1 nucleoprotein (mutant Lys164Ala) in an antiparallel configuration EMDB-67470, PDB-21al:  EMDB-67471: Asymmetric tetrameric complex of the Borna disease virus 1 nucleoprotein (mutant Arg341Ala)  EMDB-67472: Tetrameric complexes of the Borna disease virus 1 nucleoprotein (mutant Arg341Ala) in an antiparallel configuration  EMDB-67473: Hexameric complex of the Borna disease virus 1 nucleoprotein bound to RNA (mutant Arg341Ala)  EMDB-67474: Heptameric complex of the Borna disease virus 1 nucleoprotein bound to RNA (mutant Arg341Ala) |
| 由来 |
|
 キーワード | VIRAL PROTEIN / nucleoprotein / complex / VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA complex |
borna disease virus 1 (ウイルス)
escherichia coli (大腸菌)