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TitleStructural insight into PIF6-mediated red light signal transduction of plant phytochrome B.
Journal, issue, pagesCell Discov, Vol. 11, Issue 1, Page 51, Year 2025
Publish dateMay 22, 2025
AuthorsHanli Jia / Zeyuan Guan / Junya Ding / Xiaoyu Wang / Dingfang Tian / Yan Zhu / Delin Zhang / Zhu Liu / Ling Ma / Ping Yin /
PubMed AbstractThe red/far-red light receptor phytochrome B (phyB) plays essential roles in regulating various plant development processes. PhyB exists in two distinct photoreversible forms: the inactive Pr form ...The red/far-red light receptor phytochrome B (phyB) plays essential roles in regulating various plant development processes. PhyB exists in two distinct photoreversible forms: the inactive Pr form and the active Pfr form. phyB-Pfr binds phytochrome-interacting factors (PIFs) to transduce red light signals. Here, we determined the cryo-electron microscopy (cryo-EM) structures of the photoactivated phyB-Pfr‒PIF6 complex, the constitutively active mutant phyB‒PIF6 complex, and the truncated phyBN‒PIF6 complex. In these structures, two parallel phyB-Pfr molecules interact with one PIF6 molecule. Red light-triggered rotation of the PΦB D-ring leads to the conversion of hairpin loops into α helices and the "head-to-head" reassembly of phyB-Pfr N-terminal photosensory modules. The interaction between phyB-Pfr and PIF6 influences the dimerization and transcriptional activation activity of PIF6, and PIF6 stabilizes the N-terminal extension of phyB-Pfr and increases the Pr→Pfr photoconversion efficiency of phyB. Our findings reveal the molecular mechanisms underlying Pr→Pfr photoconversion and PIF6-mediated red light signal transduction of phyB.
External linksCell Discov / PubMed:40404641 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.0 Å
Structure data

60816

9irk

EMDB-60816, PDB-9irk:
Cryo-EM structure of PhyB(Y276H,1-908)-PIF6beta complex
Method: EM (single particle) / Resolution: 2.8 Å

60860

9itf

EMDB-60860, PDB-9itf:
Cryo-EM structure of full-length phyB(Y276H)-PIF6beta complex
Method: EM (single particle) / Resolution: 2.9 Å

61582

9jlb

EMDB-61582, PDB-9jlb:
Cryo-EM structure of phyB-PIF6beta complex
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-O6E:
3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid

Source
  • arabidopsis thaliana (thale cress)
KeywordsGENE REGULATION / PIF6-mediated / red light / signal transduction / phytochrome B

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