EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Chaperonin TRiC bridges radial spokes for folding locally translated proteins to sustain mammalian sperm flagellar motility.
ジャーナル・号・ページ	Mol Cell, Vol. 86, Issue 7, Page 1327-1344.e10, Year 2026
掲載日	2026年4月2日
著者	Xueming Meng / Luan Li / Cheng Lin / Yun Zhu / Yanyan Feng / Qun Zhao / Nan Zhao / Xuehai Zhou / Yujie Tong / Shanshan Wang / Guoliang Yin / Ruimin Liu / Lihua Zhang / Fei Sun / Xiumin Yan / Xueliang Zhu / Yao Cong /
PubMed 要旨	As animals evolved from external to internal fertilization, sperm flagella, once transiently propelling sperm in water to reach nearby eggs, developed to beat for days in the viscous female ...As animals evolved from external to internal fertilization, sperm flagella, once transiently propelling sperm in water to reach nearby eggs, developed to beat for days in the viscous female reproductive tract. How flagella are remodeled accordingly remains unclear. Unlike externally fertilizing zebrafish and sea urchins, mammalian flagella feature a barrel between radial spokes (RSs) RS1 and RS2. Here, we show that this RS1-RS2 barrel (RRB) is a unique T-complex protein-1 ring complex (TRiC) that folds locally translated polypeptides to sustain flagellar motility. Cryo-electron microscopy (cryo-EM) reveals a flagellum-specific TRiC structure. An in situ cryo-electron tomography (cryo-ET) map of flagellar axonemes captures the RRB TRiC in an active, substrate-receptive state, with additional densities suggestive of folding substrates and cofactors. Mammalian flagella contain components of translation machineries and locally synthesize proteins. Cross-linking mass spectrometry identifies candidate locally translated axonemal proteins and folding substrates. Furthermore, a TRiC ATPase inhibitor markedly represses mouse sperm motility. Our findings provide insights into flagellar remodeling in internally fertilizing species.
リンク	Mol Cell / PubMed:41932310
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	3.39 - 23.0 Å
構造データ	EMDB-61220: In situ structure of radial spoke 1 head in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 13.0 Å EMDB-61221: In situ structure of radial spoke 1 base in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 13.51 Å EMDB-61222: In situ structure of radial spoke 2 head in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 11.71 Å EMDB-61223: In situ structure of radial spoke 2 base in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 11.93 Å EMDB-61224: In situ structure of radial spoke 3 head in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 13.95 Å EMDB-61225: In situ structure of radial spoke 3 base in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 12.35 Å EMDB-62230: TRiC purified from bovine sperm flagella, concensus map 手法: EM (単粒子) / 解像度: 3.59 Å EMDB-62231: TRiC purified from bovine flagellar, focused refinement on E-domain 手法: EM (単粒子) / 解像度: 3.39 Å EMDB-62236: TRiC from bovine flagella, focused refinement on one-ring 手法: EM (単粒子) / 解像度: 4.14 Å 62249 9kcf EMDB-62249, PDB-9kcf: Bovine Flagellar TRiC 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-68674: Composite map of in situ structure of the 96-nm repeat DMT in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 12.0 Å EMDB-68702: Consensus map of in situ structure of the 96-nm repeat DMT in the axoneme of mouse sperm 手法: EM (サブトモグラム平均) / 解像度: 23.0 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子*YM
由来	Mus musculus (ハツカネズミ) bos taurus (ウシ)
キーワード	CHAPERONE / TRiC / sperm / flagella / motile cilia