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- EMDB-62249: Bovine Flagellar TRiC -

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Basic information

Entry
Database: EMDB / ID: EMD-62249
TitleBovine Flagellar TRiC
Map dataBovine-Flagellar-TRiC-composite-map
Sample
  • Complex: TRiC purified from bovine sperm flagella
    • Protein or peptide: x 9 types
  • Ligand: x 2 types
KeywordsTRiC / sperm / flagella / motile cilia / CHAPERONE
Function / homology
Function and homology information


Association of TriC/CCT with target proteins during biosynthesis / RHOBTB2 GTPase cycle / RHOBTB1 GTPase cycle / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / scaRNA localization to Cajal body / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / binding of sperm to zona pellucida / Neutrophil degranulation ...Association of TriC/CCT with target proteins during biosynthesis / RHOBTB2 GTPase cycle / RHOBTB1 GTPase cycle / zona pellucida receptor complex / positive regulation of establishment of protein localization to telomere / scaRNA localization to Cajal body / positive regulation of telomerase RNA localization to Cajal body / chaperonin-containing T-complex / binding of sperm to zona pellucida / Neutrophil degranulation / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / WD40-repeat domain binding / chaperone-mediated protein complex assembly / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / beta-tubulin binding / positive regulation of telomere maintenance via telomerase / mRNA 3'-UTR binding / ATP-dependent protein folding chaperone / mRNA 5'-UTR binding / response to virus / : / melanosome / G-protein beta-subunit binding / protein folding / cell body / microtubule / protein stabilization / ubiquitin protein ligase binding / centrosome / ATP hydrolysis activity / ATP binding / metal ion binding / identical protein binding
Similarity search - Function
T-complex protein 1, alpha subunit / T-complex protein 1, eta subunit / T-complex protein 1, theta subunit / T-complex protein 1, zeta subunit / T-complex protein 1, delta subunit / T-complex protein 1, gamma subunit / T-complex protein 1, epsilon subunit / T-complex protein 1, beta subunit / : / Chaperonins TCP-1 signature 1. ...T-complex protein 1, alpha subunit / T-complex protein 1, eta subunit / T-complex protein 1, theta subunit / T-complex protein 1, zeta subunit / T-complex protein 1, delta subunit / T-complex protein 1, gamma subunit / T-complex protein 1, epsilon subunit / T-complex protein 1, beta subunit / : / Chaperonins TCP-1 signature 1. / : / Chaperonins TCP-1 signature 2. / Chaperonin TCP-1, conserved site / Chaperonins TCP-1 signature 3. / Chaperone tailless complex polypeptide 1 (TCP-1) / GroEL-like equatorial domain superfamily / TCP-1-like chaperonin intermediate domain superfamily / GroEL-like apical domain superfamily / TCP-1/cpn60 chaperonin family / Chaperonin Cpn60/GroEL/TCP-1 family
Similarity search - Domain/homology
T-complex protein 1 subunit epsilon / T-complex protein 1 subunit eta / T-complex protein 1 subunit delta / T-complex protein 1 subunit alpha / T-complex protein 1 subunit zeta / Probable T-complex protein 1 subunit zeta-2 / T-complex protein 1 subunit gamma / T-complex protein 1 subunit beta / T-complex protein 1 subunit theta
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsCong Y / Meng X
Funding support China, 1 items
OrganizationGrant numberCountry
Chinese Academy of Sciences China
CitationJournal: Mol Cell / Year: 2026
Title: Chaperonin TRiC bridges radial spokes for folding locally translated proteins to sustain mammalian sperm flagellar motility.
Authors: Xueming Meng / Luan Li / Cheng Lin / Yun Zhu / Yanyan Feng / Qun Zhao / Nan Zhao / Xuehai Zhou / Yujie Tong / Shanshan Wang / Guoliang Yin / Ruimin Liu / Lihua Zhang / Fei Sun / Xiumin Yan / ...Authors: Xueming Meng / Luan Li / Cheng Lin / Yun Zhu / Yanyan Feng / Qun Zhao / Nan Zhao / Xuehai Zhou / Yujie Tong / Shanshan Wang / Guoliang Yin / Ruimin Liu / Lihua Zhang / Fei Sun / Xiumin Yan / Xueliang Zhu / Yao Cong /
Abstract: As animals evolved from external to internal fertilization, sperm flagella, once transiently propelling sperm in water to reach nearby eggs, developed to beat for days in the viscous female ...As animals evolved from external to internal fertilization, sperm flagella, once transiently propelling sperm in water to reach nearby eggs, developed to beat for days in the viscous female reproductive tract. How flagella are remodeled accordingly remains unclear. Unlike externally fertilizing zebrafish and sea urchins, mammalian flagella feature a barrel between radial spokes (RSs) RS1 and RS2. Here, we show that this RS1-RS2 barrel (RRB) is a unique T-complex protein-1 ring complex (TRiC) that folds locally translated polypeptides to sustain flagellar motility. Cryo-electron microscopy (cryo-EM) reveals a flagellum-specific TRiC structure. An in situ cryo-electron tomography (cryo-ET) map of flagellar axonemes captures the RRB TRiC in an active, substrate-receptive state, with additional densities suggestive of folding substrates and cofactors. Mammalian flagella contain components of translation machineries and locally synthesize proteins. Cross-linking mass spectrometry identifies candidate locally translated axonemal proteins and folding substrates. Furthermore, a TRiC ATPase inhibitor markedly represses mouse sperm motility. Our findings provide insights into flagellar remodeling in internally fertilizing species.
History
DepositionNov 1, 2024-
Header (metadata) releaseApr 29, 2026-
Map releaseApr 29, 2026-
UpdateApr 29, 2026-
Current statusApr 29, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_62249.png

Downloads & links

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Map

FileDownload / File: emd_62249.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationBovine-Flagellar-TRiC-composite-map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.87 Å/pix.
x 400 pix.
= 346.28 Å		0.87 Å/pix.
x 400 pix.
= 346.28 Å		0.87 Å/pix.
x 400 pix.
= 346.28 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8657 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 2.5
Minimum - Maximum0.0 - 15.250026999999999
Average (Standard dev.)0.096190795 (±0.6920087)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 346.28 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : TRiC purified from bovine sperm flagella

EntireName: TRiC purified from bovine sperm flagella
Components
  • Complex: TRiC purified from bovine sperm flagella
    • Protein or peptide: T-complex protein 1 subunit alpha
    • Protein or peptide: T-complex protein 1 subunit beta
    • Protein or peptide: T-complex protein 1 subunit gamma
    • Protein or peptide: T-complex protein 1 subunit delta
    • Protein or peptide: T-complex protein 1 subunit epsilon
    • Protein or peptide: T-complex protein 1 subunit zeta
    • Protein or peptide: T-complex protein 1 subunit eta
    • Protein or peptide: T-complex protein 1 subunit theta
    • Protein or peptide: Probable T-complex protein 1 subunit zeta-2
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-DIPHOSPHATE

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Supramolecule #1: TRiC purified from bovine sperm flagella

SupramoleculeName: TRiC purified from bovine sperm flagella / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #3-#7, #9, #2, #8, #1
Source (natural)Organism: Bos taurus (domestic cattle)

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Macromolecule #1: Probable T-complex protein 1 subunit zeta-2

MacromoleculeName: Probable T-complex protein 1 subunit zeta-2 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 58.114383 KDa
SequenceString: MAAIKAINSK AEVARAQAAL AVNICAARGL QDVLRTNLGP KGTMKMLVSG AGDVKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITG DGTTSNVLII GELLKQADLY ISEGLHPRII AEGFEIAKIK ALEVLEQVKI KKEMKREIHL DVARTSLQTK V HPQLADVL ...String:
MAAIKAINSK AEVARAQAAL AVNICAARGL QDVLRTNLGP KGTMKMLVSG AGDVKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITG DGTTSNVLII GELLKQADLY ISEGLHPRII AEGFEIAKIK ALEVLEQVKI KKEMKREIHL DVARTSLQTK V HPQLADVL TEAVVDSVLA IRRPNYPIDL FMVEIMEMKH KSETDTKLIK GLVLDHGARH PDMKKRVDDA FILTCNVSLE YE KTEVSSG FFYKTAEEKE KLVKAERKFI EDRVQKIIDL KDKVCAQSNK GFVVINQKGI DPFSLDALAK HGILALRRAK RRN MERLSL ACGGVAVNSV EDLSVDCLGH AGLVYEYTLG EEKYTFIEDC INPRSVTLLV KGPNKHTLTQ IKDAVRDGLR AIKN AIEDG CVVPGAGAVE VVIAEALVTY KHTIQGRARL GVQAFADALL IIPKVLAQNS GYDLQETLVK VQAEHSNSKQ PVGID LNTG EPMVAADAGV WDNYCVKKQL LHSCTVIATN ILLVDEIMRA GMSSLKG

UniProtKB: Probable T-complex protein 1 subunit zeta-2

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Macromolecule #2: T-complex protein 1 subunit eta

MacromoleculeName: T-complex protein 1 subunit eta / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 59.518707 KDa
SequenceString: MMPTPVILLK EGTDSSQGIP QLVSNISACQ VIAEAVRTTL GPRGMDKLIV DGRGKATISN DGATILKLLD VVHPAAKTLV DIAKSQDAE VGDGTTSVTL LAAEFLKQVK PYVEEGLHPQ IIIRAFRTAT QLAVNKIKEI AVTVKKEDKV EQRKLLEKCA M TALSSKLI ...String:
MMPTPVILLK EGTDSSQGIP QLVSNISACQ VIAEAVRTTL GPRGMDKLIV DGRGKATISN DGATILKLLD VVHPAAKTLV DIAKSQDAE VGDGTTSVTL LAAEFLKQVK PYVEEGLHPQ IIIRAFRTAT QLAVNKIKEI AVTVKKEDKV EQRKLLEKCA M TALSSKLI SQQKAFFAKM VVDAVMMLDD LLQLKMIGIK KVQGGALEES QLVAGVAFKK TFSYAGFEMQ PKKYHNPMIA LL NVELELK AEKDNAEIRV HTVEDYQAIV DAEWNILYDK LEKIHHSGAK VVLSKLPIGD VATQYFADRD MFCAGRVPEE DLK RTMMAC GGSIQTSVNA LSSDVLGRCQ VFEETQIGGE RYNFFTGCPK AKTCTIILRG GAEQFMEETE RSLHDAIMIV RRAI KNDSV VAGGGAIEME LSKYLRDYSR TIPGKQQLLI GAYAKALEII PRQLCDNAGF DATNILNKLR ARHAQGGMWY GVDIN TEDI ADNFEAFVWE PAMVRINALT AASEAACLIV SVDETIKNPR STVDASPAAG RGRGRGRLH

UniProtKB: T-complex protein 1 subunit eta

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Macromolecule #3: T-complex protein 1 subunit alpha

MacromoleculeName: T-complex protein 1 subunit alpha / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 60.281312 KDa
SequenceString: MEGPLSVFGD RSTGEAIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDG TTSVVIIAAE LLKNADELVK QKIHPTSVIS GYRLACKEAV RYISENLIIN TDELGRDCLI NAAKTSMSSK V IGINGDFF ...String:
MEGPLSVFGD RSTGEAIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDG TTSVVIIAAE LLKNADELVK QKIHPTSVIS GYRLACKEAV RYISENLIIN TDELGRDCLI NAAKTSMSSK V IGINGDFF ANLVVDAVLA IKYTDIRGQP RYPVNSINVL KAHGRSQMES MLINGYALNC VVGSQGMPKR IVNAKIACLD FS LQKTKMK LGVQVVITDP EKLDQIRQRE SDITKERIQK ILATGANVIL TTGGIDDMCL KYFVEAGAMA VRRVLKRDLK RIA KASGAT VLSTLANLEG EETFEASMLG QAEEVVQERI CDDELILIKN TKARTSASVI LRGANDFMCD EMERSLHDAL CVVK RVLES KSVVPGGGAV EAALSIYLEN YATSMGSREQ LAIAEFARSL PVIPNTLAVN AAQDSTDLVA KLRAFHNEAQ VNPER KNLK WIGLDLVNGK PRDNKQAGVF EPTIVKVKSL KFATEAAITI LRIDDLIKLH PESKDDKHGG YEDAVHSGAL DA

UniProtKB: T-complex protein 1 subunit alpha

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Macromolecule #4: T-complex protein 1 subunit beta

MacromoleculeName: T-complex protein 1 subunit beta / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 57.555188 KDa
SequenceString: MASLSLAPVN IFKAGADEER AETARLSSFI GAIAIGDLVK STLGPKGMDK ILLSSGRDAS LMVTNDGATI LKNIGVDNPA AKVLVDMSR VQDDEVGDGT TSVTVLAAEL LREAESLIAK KIHPQTIIAG WREATKAARQ ALLNSAVDHG SDEVKFRQDL M NIAGTTLS ...String:
MASLSLAPVN IFKAGADEER AETARLSSFI GAIAIGDLVK STLGPKGMDK ILLSSGRDAS LMVTNDGATI LKNIGVDNPA AKVLVDMSR VQDDEVGDGT TSVTVLAAEL LREAESLIAK KIHPQTIIAG WREATKAARQ ALLNSAVDHG SDEVKFRQDL M NIAGTTLS SKLLTHHKDH FTKLAVEAVL RLKGSGNLEA IHVIKKLGGS LADSYLDEGF LLDKKIGVNQ PKRIENAKIL IA NTGMDTD KIKIFGSRVR VDSTAKVAEI EHAEKEKMKE KVERILKHGI NCFINRQLIY NYPEQLFGAA GVMAIEHADF VGV ERLALV TGGEIASTFD HPELVKLGSC KLIEEVMIGE DKLIHFSGVA LGEACTIVLR GATQQILDEA ERSLHDALCV LAQT VKDSR TVYGGGCSEM LMAHAVTQLA SRTPGKEAVA MESYAKALRM LPTIIADNAG YDSADLVAQL RAAHSEGKTT AGLDM KEGT IGDMSVLGIT ESFQVKRQVL LSAAEAAEVI LRVDNIIKAA PRKRVPDHHP C

UniProtKB: T-complex protein 1 subunit beta

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Macromolecule #5: T-complex protein 1 subunit gamma

MacromoleculeName: T-complex protein 1 subunit gamma / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 60.666848 KDa
SequenceString: MMGHRPVLVL SQNTKRESGR KVQSGNINAA KTIADIIRTC LGPKSMMKML LDPMGGIVMT NDGNAILREI QVQHPAAKSM IEISRTQDE EVGDGTTSVI ILAGEMLSVA EHFLEQQMHP TVVISAYRKA LDDMISTLKK ISIPVDTSNR DTMLNIINSS I TTKVISRW ...String:
MMGHRPVLVL SQNTKRESGR KVQSGNINAA KTIADIIRTC LGPKSMMKML LDPMGGIVMT NDGNAILREI QVQHPAAKSM IEISRTQDE EVGDGTTSVI ILAGEMLSVA EHFLEQQMHP TVVISAYRKA LDDMISTLKK ISIPVDTSNR DTMLNIINSS I TTKVISRW SSLACNIALD AVKTVQFEEN GRKEIDIKKY ARVEKIPGGI IEDSCVLRGV MINKDVTHPR MRRYIKNPRI VL LDSSLEY KKGESQTDIE ITREEDFTRI LQMEEEYIQQ LCEDIIQLKP DVVITEKGIS DLAQHYLMRA NITAIRRVRK TDN NRIARA CGARIVSRPE ELREEDVGTG AGLLEIKKIG DEYFTFITEC KDPKACTILL RGASKEILSE VERNLQDAMQ VCRN VLLDP QLVPGGGASE MAVAHALTEK SKAMTGVEQW PYRAVAQALE VIPRTLIQNC GASTIRLLTS LRAKHTQENC ETWGV NGET GTLVDMKELG IWEPLAVKLQ TYKTAVETAV LLLRIDDIVS GHKKKGDDQS RQGGAPDAGQ E

UniProtKB: T-complex protein 1 subunit gamma

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Macromolecule #6: T-complex protein 1 subunit delta

MacromoleculeName: T-complex protein 1 subunit delta / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 58.278355 KDa
SequenceString: MPENVAPRTG PPAGAAGAAG GRGKSAYQDR DKPAQIRFSN ISAAKAVADA IRTSLGPKGM DKMIQDGKGD VTITNDGATI LKQMQVLHP AARMLVELSK AQDIEAGDGT TSVVIIAGSL LDSCTKLLQK GIHPTIISES FQKALEKGIE ILTDMSRPEE L SDRETLLN ...String:
MPENVAPRTG PPAGAAGAAG GRGKSAYQDR DKPAQIRFSN ISAAKAVADA IRTSLGPKGM DKMIQDGKGD VTITNDGATI LKQMQVLHP AARMLVELSK AQDIEAGDGT TSVVIIAGSL LDSCTKLLQK GIHPTIISES FQKALEKGIE ILTDMSRPEE L SDRETLLN SAATSLNSKV VSQYSSLLSP MSVDAVMKVI DPATATSVDL RDIKIVKKLG GTIDDCELVE GLVLTQKVAN SG ITRVEKA KIGLIQFCLS APKTDMDNQI VVSDYVQMDR VLREERAYIL NLVKQIKKTG CNVLLIQKSI LRDALSDLAL HFL NKMKIM VVKDIEREDI EFICKTIGTK PVAHVDQFTA DMLGSAELAE EVSLNGSGKL IKITGCASPG KTVTIVVRGS NKLV IEEAE RSIHDALCVI RCLVKKRALI AGGGAPEIEL ALRLTEYSRT LSGMESYCIR AFADAMEVIP STLAENAGLN PISTV TELR NRHAQGEKTT GINVRKGGIS NILEEQVVQP LLVSVSALTL ATETVRSILK IDDVVNTR

UniProtKB: T-complex protein 1 subunit delta

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Macromolecule #7: T-complex protein 1 subunit epsilon

MacromoleculeName: T-complex protein 1 subunit epsilon / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 59.681941 KDa
SequenceString: MASVGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMKT SLGPNGLDKM MVDKDGDVTV TNDGATILSM MDVDHQIAK LMVELSKSQD DEIGDGTTGV VVLAGALLEE AEQLLDRGIH PIRIADGYEQ AARIAIEHLD KISDSVLVDM K NTEPLIQT ...String:
MASVGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMKT SLGPNGLDKM MVDKDGDVTV TNDGATILSM MDVDHQIAK LMVELSKSQD DEIGDGTTGV VVLAGALLEE AEQLLDRGIH PIRIADGYEQ AARIAIEHLD KISDSVLVDM K NTEPLIQT AKTTLGSKVV NSCHRQMAEI AVNAVLTVAD MQRRDVDFEL IKVEGKVGGR LEDTKLIKGV IVDKDFSHPQ MP KQVEDAK IAILTCPFEP PKPKTKHKLD VTSVEDFKAL QKYEKEKFEE MIRQIKETGA NLAVCQWGFD DEANHLLLQN DLP AVRWVG GPEIELIAIA TGGRIVPRFS ELTAEKLGFA GLVKEISFGT TKDKMLVIEQ CKNSRAVTIF IRGGNKMIIE EAKR SLHDA LCVIRNLIRD NRVVYGGGAA EISCALAVSQ EADKCPTLEQ YAMRAFADAL EVIPMALAEN SGMNPIQTMT EVRAR QVKE VNPALGIDCL HKGTNDMKHQ HVIETLIGKK QQISLATQMV RMILKIDDIR KPGESEE

UniProtKB: T-complex protein 1 subunit epsilon

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Macromolecule #8: T-complex protein 1 subunit theta

MacromoleculeName: T-complex protein 1 subunit theta / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 59.681328 KDa
SequenceString: MALHVPKAPG FAQMLKEGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN GMNKMVINHL EKLFVTNDAA TILRELEVQH PAAKMIVMA SHMQEQEVGD GTNFVLVFAG ALLELAEELL RLGLSVSEVI EGYEIACKKA HEILPDLVCC SAKNLRDVDE V SSLLHTSV ...String:
MALHVPKAPG FAQMLKEGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN GMNKMVINHL EKLFVTNDAA TILRELEVQH PAAKMIVMA SHMQEQEVGD GTNFVLVFAG ALLELAEELL RLGLSVSEVI EGYEIACKKA HEILPDLVCC SAKNLRDVDE V SSLLHTSV MSKQYGNEVF LAKLIAQACV SIFPDSGHFN VDNIRVCKIL GSGVHSSSVL HGMVFKKETE GDVTSVKDAK IA VYSCPFD GMITETKGTV LIKSAEELMN FSKGEENLMD AQVKAIADTG ANVVVTGGRV ADMALHYANK YNIMLVRLNS KWD LRRLCK TVGATALPRL NPPVLEEMGH CDSVYLSEVG DTQVVVFKHE KEDGAISTIV LRGSTDNLMD DIERAVDDGV NTFK VLTRD KRLVPGGGAT EIELAKQITS YGETCPGLEQ YAIKKFAEAF EAIPRALAEN SGVKANEVIS KLYAVHQEGN KNVGL DIEA EVPAVKDMLE AGVLDTYLGK YWAIKLATNA AVTVLRVDQI IMAKPAGGPK PPSGKKDWDE DQND

UniProtKB: T-complex protein 1 subunit theta

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Macromolecule #9: T-complex protein 1 subunit zeta

MacromoleculeName: T-complex protein 1 subunit zeta / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides
Source (natural)Organism: Bos taurus (domestic cattle)
Molecular weightTheoretical: 58.037086 KDa
SequenceString: MAAVKTLNPK AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITG DGTTSNVLII GELLKQADLY ISEGLHPRII TEGFEAAKEK ALQFLEQVKV SKEMDRETLI DVARTSLRTK V HAELADVL ...String:
MAAVKTLNPK AEVARAQAAL AVNISAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG NVLLHEMQIQ HPTASLIAKV ATAQDDITG DGTTSNVLII GELLKQADLY ISEGLHPRII TEGFEAAKEK ALQFLEQVKV SKEMDRETLI DVARTSLRTK V HAELADVL TEAVVDSILA IKKQDEPIDL FMVEIMEMKH KSETDTSLIR GLVLDHGARH PDMKKRVEDA YILTCNVSLE YE KTEVNSG FFYKSAEERE KLVKAERKFI EDRVKKIIEL KKKVCGDSDK GFVVINQKGI DPFSLDALAK EGIIALRRAK RRN MERLTL ACGGIALNSL DDLNPDCLGH AGLVYEYTLG EEKFTFIEKC NNPRSVTLLI KGPNKHTLTQ IKDAIRDGLR AVKN AIDDG CVVPGAGAVE VAMAEALVKY KPSVKGRAQL GVQAFADALL IIPKVLAQNS GFDLQETLVK VQAEHSESGQ LVGVD LNTG EPMVAAEAGI WDNYCVKKQL LHSCTVIATN ILLVDEIMRA GMSSLKG

UniProtKB: T-complex protein 1 subunit zeta

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Macromolecule #10: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 10 / Number of copies: 6 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #11: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 11 / Number of copies: 6 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 54.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.01 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4) / Number images used: 130150
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

Initial model
PDB IDChain

4a0v

source_name: PDB, initial_model_type: experimental model

4-f1

source_name: AlphaFold, initial_model_type: in silico model
Output model

PDB-9kcf:
Bovine Flagellar TRiC

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