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TitleMycobacteriophage cryo-EM studies reveal the siphophage architecture at the amino acid level and its host interaction for viral genome ejection
Journal, issue, pagesTo Be Published
Publish dateMay 2, 2024 (structure data deposition date)
AuthorsMaharana J / Wang CH / Tsai LA / Lowary TL / Ho MC
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.63 - 6.03 Å
Structure data

EMDB-39973, PDB-8zdh:
Cryo-EM structure of Mycobacteriophage Douge genome-packed capsid (gp8 and gp113)
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-39974: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (Penton region: gp8)
Method: EM (single particle) / Resolution: 2.95 Å

EMDB-39975: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (P-hexon region: gp8 and gp113)
Method: EM (single particle) / Resolution: 2.83 Å

EMDB-39976: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (C-hexon region: gp8 and gp113)
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-39977: Composite cryo-EM map of Mycobacteriophage Douge genome-packed capsid (Penton, P-hexon and C-hexon region: gp8 and gp113)
Method: EM (single particle) / Resolution: 2.86 Å

EMDB-39978, PDB-8zdi:
Cryo-EM structure of Mycobacteriophage Douge genome-free capsid (gp8)
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-39979: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (Penton region: gp8)
Method: EM (single particle) / Resolution: 3.37 Å

EMDB-39980: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (P-hexon region: gp8)
Method: EM (single particle) / Resolution: 3.16 Å

EMDB-39981: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (C-hexon region: gp8)
Method: EM (single particle) / Resolution: 3.12 Å

EMDB-39982: Composite cryo-EM map of Mycobacteriophage Douge genome-free capsid (Penton, P-hexon and C-hexon region: gp8)
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-39983, PDB-8zdj:
Cryo-EM structure of Mycobacteriophage Douge genome-packed connector (gp5, gp9, gp10, gp12 and gp13)
Method: EM (single particle) / Resolution: 3.74 Å

EMDB-39985: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp5 and gp9)
Method: EM (single particle) / Resolution: 2.82 Å

EMDB-39986: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp10, gp12 and gp13)
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-39987: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp8 and gp113)
Method: EM (single particle) / Resolution: 3.24 Å

EMDB-39988: Composite cryo-EM map of Mycobacteriophage Douge genome-packed connector-vertex (gp5, gp9, gp10, gp12, gp13, gp8 and gp113)
Method: EM (single particle) / Resolution: 3.24 Å

EMDB-39989, PDB-8zdl:
Cryo-EM structure of Mycobacteriophage Douge genome-free connector (gp5, gp9, gp10, gp12 and gp13)
Method: EM (single particle) / Resolution: 3.78 Å

EMDB-39991: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp5 and gp9)
Method: EM (single particle) / Resolution: 3.53 Å

EMDB-39992: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp10, gp12 and gp13)
Method: EM (single particle) / Resolution: 3.89 Å

EMDB-39993: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp8)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-39994: Composite cryo-EM map of Mycobacteriophage Douge genome-free connector-vertex (gp5, gp9, gp10, gp12, gp13 and gp8)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-39995, PDB-8zdn:
Cryo-EM structure of Mycobacteriophage Douge genome-free tail tube (gp13)
Method: EM (single particle) / Resolution: 3.07 Å

EMDB-39996, PDB-8zdo:
Cryo-EM structure of Mycobacteriophage Douge baseplate (gp13, gp17, gp23, gp16, gp18 and gp20)
Method: EM (single particle) / Resolution: 2.97 Å

EMDB-39997: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp13)
Method: EM (single particle) / Resolution: 2.71 Å

EMDB-39998: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp17 and gp23)
Method: EM (single particle) / Resolution: 2.75 Å

EMDB-39999: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp16, gp18 and gp20)
Method: EM (single particle) / Resolution: 2.63 Å

EMDB-60000: Composite cryo-EM map of Mycobacteriophage Douge baseplate (gp13, gp17, gp23, gp16, gp18 and gp20)
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-60001, PDB-8zdp:
Cryo-EM structure of Mycobacteriophage Douge Central fiber (gp20)
Method: EM (single particle) / Resolution: 4.26 Å

EMDB-60002: Focused refinement cryo-EM map of Mycobacteriophage Douge Central fiber (gp20, top region)
Method: EM (single particle) / Resolution: 4.48 Å

EMDB-60003: Focused refinement cryo-EM map of Mycobacteriophage Douge central fiber (gp20, bottom region)
Method: EM (single particle) / Resolution: 3.88 Å

EMDB-60004: Composite cryo-EM map of Mycobacteriophage Douge central fiber (gp20)
Method: EM (single particle) / Resolution: 4.18 Å

EMDB-60005, PDB-8zdq:
Cryo-EM structure of Mycobacteriophage Douge complete baseplate (gp13, gp17, gp23, gp16, gp18 and gp20)
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-60714: Cryo-EM structure of Mycobacteriophage Douge genome-packed connector-vertex (gp5, gp8, gp9, gp10, gp12, gp13 and gp113
Method: EM (single particle) / Resolution: 3.74 Å

EMDB-60715: Cryo-EM structure of Mycobacteriophage Douge genome-free connector vertex (gp5, gp8, gp9, gp10, gp12 and gp13)
Method: EM (single particle) / Resolution: 6.03 Å

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsVIRUS / Mycobacteriophage / phage structure / Icosahedral / cryo-EM / protein assembly / VIRAL PROTEIN

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