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TitleStructural insights into polyamine spermidine uptake by the ABC transporter PotD-PotABC.
Journal, issue, pagesSci Adv, Vol. 10, Issue 38, Page eado8107, Year 2024
Publish dateSep 20, 2024
AuthorsZhu Qiao / Phong Hoa Do / Joshua Yi Yeo / Rya Ero / Zhuowen Li / Liying Zhan / Sandip Basak / Yong-Gui Gao /
PubMed AbstractPolyamines, characterized by their polycationic nature, are ubiquitously present in all organisms and play numerous cellular functions. Among polyamines, spermidine stands out as the predominant type ...Polyamines, characterized by their polycationic nature, are ubiquitously present in all organisms and play numerous cellular functions. Among polyamines, spermidine stands out as the predominant type in both prokaryotic and eukaryotic cells. The PotD-PotABC protein complex in , belonging to the adenosine triphosphate-binding cassette transporter family, is a spermidine-preferential uptake system. Here, we report structural details of the polyamine uptake system PotD-PotABC in various states. Our analyses reveal distinct "inward-facing" and "outward-facing" conformations of the PotD-PotABC transporter, as well as conformational changes in the "gating" residues (F222, Y223, D226, and K241 in PotB; Y219 and K223 in PotC) controlling spermidine uptake. Therefore, our structural analysis provides insights into how the PotD-PotABC importer recognizes the substrate-binding protein PotD and elucidates molecular insights into the spermidine uptake mechanism of bacteria.
External linksSci Adv / PubMed:39303029 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.5 Å
Structure data

38933

8y5f

EMDB-38933, PDB-8y5f:
Cryo-EM structure of E.coli spermidine transporter PotABC
Method: EM (single particle) / Resolution: 3.13 Å

38934

8y5g

EMDB-38934, PDB-8y5g:
Cryo-EM structure of E.coli spermidine transporter PotABC with spermidine
Method: EM (single particle) / Resolution: 3.0 Å

38935

8y5h

EMDB-38935, PDB-8y5h:
Cryo-EM structure of E.coli spermidine transporter PotD-PotABC in pre-translocation state
Method: EM (single particle) / Resolution: 3.1 Å

38936

8y5i

EMDB-38936, PDB-8y5i:
Cryo-EM structure of E.coli spermidine transporter PotD-PotABC in translocation intermidiate state
Method: EM (single particle) / Resolution: 3.0 Å

60536

8zx1

EMDB-60536, PDB-8zx1:
Cryo-EM structure of E.coli spermidine transporter PotABC in nanodisc
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-SPD:
SPERMIDINE

ChemComp-HOH:
WATER

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • escherichia coli (E. coli)
KeywordsTRANSPORT PROTEIN / ABC transporter

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