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TitleStructural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1.
Journal, issue, pagesPlant Commun, Vol. 6, Issue 1, Page 101181, Year 2025
Publish dateJan 13, 2025
AuthorsHong Wei / Heyuan Zhu / Wei Ying / Hilde Janssens / Miroslav Kvasnica / Johan M Winne / Yongxiang Gao / Jiří Friml / Qian Ma / Shutang Tan / Xin Liu / Eugenia Russinova / Linfeng Sun /
PubMed AbstractBrassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for ...Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signaling initiation, which requires binding of a BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter ABCB19 functions as a BR exporter and, together with its close homolog ABCB1, positively regulates BR signaling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 can be stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 were determined in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states. In the BL-bound structure, BL is bound to the hydrophobic cavity formed by the transmembrane domain and triggers local conformational changes. Together, our data provide additional insights into ABC transporter-mediated BR export.
External linksPlant Commun / PubMed:39497419 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 3.8 Å
Structure data

60366

8zpx

EMDB-60366, PDB-8zpx:
Structure of the wild-type Arabidopsis ABCB1 in the apo state
Method: EM (single particle) / Resolution: 3.5 Å

60369

8zpz

EMDB-60369, PDB-8zpz:
Structure of the wild-type Arabidopsis ABCB1 in the brassinolide-bound state
Method: EM (single particle) / Resolution: 3.5 Å

60370

8zq4

EMDB-60370, PDB-8zq4:
Structure of the Arabidopsis ABCB1 EQ mutant in the BL plus ATP bound state
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-BLD:
Brassinolide

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / ABC transporter / wild-type / apo state / brassinolide-bound state

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