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-Structure paper
| タイトル | Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1. |
|---|---|
| ジャーナル・号・ページ | Plant Commun, Vol. 6, Issue 1, Page 101181, Year 2025 |
| 掲載日 | 2025年1月13日 |
著者 | Hong Wei / Heyuan Zhu / Wei Ying / Hilde Janssens / Miroslav Kvasnica / Johan M Winne / Yongxiang Gao / Jiří Friml / Qian Ma / Shutang Tan / Xin Liu / Eugenia Russinova / Linfeng Sun / ![]() |
| PubMed 要旨 | Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for ...Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signaling initiation, which requires binding of a BR molecule to the extracellular domains of the plasma membrane-localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter ABCB19 functions as a BR exporter and, together with its close homolog ABCB1, positively regulates BR signaling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 can be stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 were determined in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states. In the BL-bound structure, BL is bound to the hydrophobic cavity formed by the transmembrane domain and triggers local conformational changes. Together, our data provide additional insights into ABC transporter-mediated BR export. |
リンク | Plant Commun / PubMed:39497419 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.5 - 3.8 Å |
| 構造データ | EMDB-60366, PDB-8zpx: EMDB-60369, PDB-8zpz: EMDB-60370, PDB-8zq4: |
| 化合物 | ![]() ChemComp-BLD: ![]() ChemComp-ATP: |
| 由来 |
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キーワード | TRANSPORT PROTEIN / ABC transporter / wild-type / apo state / brassinolide-bound state |
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